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Methods for hatching margined madtom eggs

机译:孵化边缘淡淡蛋的方法

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We used the margined madtom Noturus insignis as a model species to develop egg-hatching techniques for other madtom species. Twelve methods were tested at water temperatures between 22 deg C and 30 deg C, but only one method produced consistent, high hatches of madtom eggs. Use of broodfish to incubate eggs was not effective because they ate their tank-spawned egg masses. Consequently, madtom eggs must be artificially incubated. Use of sodium sulfite, Na_2SO_3, to facilitate artificial incubation by separating madtom egg masses is not effective because it causes premature hatching and subsequent low survival of larvae. Agitation strong enough to tumble eggs and high temperature appear to be key in consistently producing good hatches. We obtained hatch rates greater than 65% for 14 of 16 margined madtom egg masses by incubating them at 28-30 deg C within a 6-mm-bar-mesh plastic basket positioned over an air steam strong enough to keep egg masses in constant motion. The larvae hatched in about 7 d at 28 deg C, and the mean number of degree-days for hatching was 205 +- 14 (N = 6).
机译:我们将边缘的madtom Noturus insignis用作模型物种,为其他madtom物种开发卵孵化技术。在水温22摄氏度至30摄氏度之间测试了十二种方法,但只有一种方法可产生一致的高孵化率的madtom卵。使用亲鱼孵化卵是无效的,因为它们吃掉了坦克产卵的卵团。因此,madtom卵必须人工孵育。使用亚硫酸钠Na_2SO_3来通过分离madtom卵团来促进人工孵化是无效的,因为它会导致过早孵化并导致幼虫的低存活率。足以搅动鸡蛋和高温的搅动似乎是持续产生良好孵化的关键。我们通过在28 mm至30℃的6毫米长网眼塑料篮中将其孵化,孵化率超过16个边缘madtom蛋块中的14个,其孵化率要强于足以保持蛋块恒定运动的空气蒸汽。幼虫在28摄氏度约7 d内孵化,孵化的平均天数为205±14(N = 6)。

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