...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic Acids Research >Structure-dependent reactivity of oxyfunctionalized acetophenones in the photooxidation of DNA: base oxidation and strand breaks through photolytic radical formation (spin trapping, EPR spectroscopy, transient kinetics) versus photosensitization (ele
【24h】

Structure-dependent reactivity of oxyfunctionalized acetophenones in the photooxidation of DNA: base oxidation and strand breaks through photolytic radical formation (spin trapping, EPR spectroscopy, transient kinetics) versus photosensitization (ele

机译：氧官能化的苯乙酮在DNA的光氧化中的结构依赖性反应性：通过光解自由基的形成（自旋捕集，EPR光谱，瞬态动力学）与光敏化（ele）进行碱基氧化和链断裂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The photooxidative damage of DNA, specifically guanine oxidation and strand-break formation, by sidechain-oxyfunctionalized acetophenones (hydroxy, methoxy, tert-butoxy and acetoxy derivatives), has been examined. The involvement of triplet-excited ketones and their reactivity towards DNA has been determined by time-resolved laser-flash spectro-scopy. The generation of carbon-centered radical species upon Norrish-type I cleavage has been assessed by spin-trapping experiments with 5,5-dimethyl-1-pyrroline N-oxide, coupled with electron paramagnetic resonance spectroscopy. The observed DNA-base oxidation and strand-break formation is discussed in terms of the peroxyl radicals derived from the triplet-excited ketones by α cleavage and molecular oxygen trapping, as well as direct interaction of the excited states by electron transfer and hydrogen-atom abstraction. It is concluded that acetophenone derivatives, which produce radicals upon photolysis, in particular the hydroxy (AP-OH) and tert-butoxy (AP-O~tBu) derivatives, are more effective in oxidizing DNA.
机译：研究了侧链-氧官能化的苯乙酮（羟基，甲氧基，叔丁氧基和乙酰氧基衍生物）对DNA的光氧化损伤，特别是鸟嘌呤氧化和链断裂的形成。三重激发态酮的参与及其对DNA的反应性已通过时间分辨激光闪光光谱法确定。通过使用5，5-二甲基-1-吡咯啉N-氧化物与电子顺磁共振波谱的自旋俘获实验，评估了Norrish I型裂解后以碳为中心的自由基物种的产生。讨论了观察到的DNA碱基的氧化和链断裂的形成，讨论了三重激发的酮通过α裂解和分子氧陷阱衍生的过氧自由基，以及电子转移和氢原子激发态的直接相互作用。抽象。结论是在光解时产生自由基的苯乙酮衍生物，特别是羟基（AP-OH）和叔丁氧基（AP-O〜tBu）衍生物，在氧化DNA方面更有效。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号