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首页> 外文期刊>Nucleic Acids Research >The severe slow growth of Δsrs2 Δrqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes
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The severe slow growth of Δsrs2 Δrqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes

机译：重组酵母和检查点基因的丢失抑制了粟酒裂殖酵母中Δsrs2Δrqh1的严重缓慢生长

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摘要

Our interest in the Schizosaccharomyces pombe RecQ helicase, rqh1~+, led us to investigate the function of a related putative DNA helicase, srs2~+. We identified the srs2~+ homolog in S. pombe, and found that srs2~+ is not essential for cell viability. A Δsrs2 Δrqh1 double mutant grows extremely slowly with aberrant shaped cells and low viability. This slow growth does not appear to be related to stalled replication, as Δsrs2 Δrqh1 cells showed higher survival rates, compared with Δrqh1, when stalled forks were increased by UV irradiation or hydroxy urea treatment. Consistent with this result, we found that Δsrs2 Δrqh1 cells progress through S-phase with a slight delay, but undergo a checkpoint-dependent arrest presumably at G_2/M. Further, we found that Δsrs2 Δrqh1 slow growth is related to recombination, as loss of either the rhp51~+ or rhp57~+ recombination genes improves cell growth in the double mutant. Δsrs2 is also synthetic lethal with Δrhp54, another homologous recombination gene. This lethality is suppressed in a Δrhp51 background. Together, these results demonstrate a clear genetic interaction between rqh1~+, srs2~+ and the genes of the homologous recombination pathway.
机译：我们对粟酒裂殖酵母RecQ解旋酶rqh1〜+的兴趣促使我们研究了相关推定DNA解旋酶srs2〜+的功能。我们在粟酒裂殖酵母中鉴定出srs2〜+同源物，发现srs2〜+对于细胞活力不是必需的。 Δsrs2Δrqh1双突变体的生长异常缓慢，形状细胞异常且活力较低。这种缓慢的增长似乎与停滞的复制无关，因为当通过紫外线照射或羟基脲处理增加停滞的叉子时，与Δrqh1相比，Δsrs2Δrqh1细胞显示出更高的存活率。与此结果一致，我们发现Δsrs2Δrqh1细胞略有延迟地通过S期，但可能在G_2 / M处经历了依赖检查点的逮捕。此外，我们发现Δsrs2Δrqh1的缓慢生长与重组有关，因为rhp51〜+或rhp57〜+重组基因的缺失会改善双突变体中的细胞生长。 Δsrs2也是与另一个同源重组基因Δrhp54合成致死的。这种杀伤力在Δrhp51背景下得到抑制。总之，这些结果表明rqh1〜+，srs2〜+与同源重组途径的基因之间存在清晰的遗传相互作用。

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