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首页> 外文期刊>Nucleic Acids Research >Specific roles of 5' RNA secondary structures in stabilizing transcripts in chloroplasts.
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Specific roles of 5' RNA secondary structures in stabilizing transcripts in chloroplasts.

机译：5'RNA二级结构在稳定叶绿体中转录本中的特定作用。

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摘要

RNA secondary structures, e.g. stem-loops that are often found at the 5' and 3' ends of mRNAs, are in many cases known to be crucial for transcript stability but their role in prolonging the lifetime of transcripts remains elusive. In this study we show for an essential RNA-stabilizing stem-loop at the 5' end of rbcL gene transcripts in Chlamydomonas that it neither prevents ribonucleases from binding to the RNA nor impedes their movement along the RNA strand. The stem-loop has a formative function in that it mediates folding of a short sequence around its base into a specific RNA conformation, consisting of a helical and single-stranded region, i.e. the real structure required for longevity of rbcL transcripts in chloroplasts. Disturbing this structure renders transcripts completely unstable, even if the sequence of this element is not altered. The requirement of a specific 5' sequence and structure for RNA longevity suggests an interaction of this element with a trans-acting factor that protects transcripts from rapid degradation in chloroplasts.
机译：RNA二级结构，例如通常在mRNA的5'和3'末端发现茎环，在许多情况下，它们对于转录物的稳定性至关重要，但是它们在延长转录物寿命中的作用仍然难以捉摸。在这项研究中，我们显示了衣藻中rbcL基因转录物5'端必需的稳定RNA的茎环，它既不会阻止核糖核酸与RNA结合，也不会阻止其沿RNA链移动。茎环具有形成功能，因为它介导围绕其碱基的短序列折叠成特定的RNA构象，该构象由螺旋和单链区域组成，即叶绿体中rbcL转录本的寿命所需的真实结构。干扰此结构会使脚本完全不稳定，即使该元素的顺序未更改也是如此。 RNA寿命需要特定的5'序列和结构，表明该元素与反式作用因子的相互作用可保护转录本免受叶绿体的快速降解。

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