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首页> 外文期刊>Nucleic Acids Research >THE SMALL SUBUNIT OF THE SPLICING FACTOR U2AF IS CONSERVED IN FISSION YEAST
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THE SMALL SUBUNIT OF THE SPLICING FACTOR U2AF IS CONSERVED IN FISSION YEAST

机译:分裂酵母中保留了拼接因子U2AF的小亚基

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The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa, Previously we identified the Schizosaccharomyces pombe homolog, spU2AF(59), of the human large subunit, We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF(59) using the yeast two-hybrid system and have identified a homolog of the hsU2AF(35) subunit, The S.pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit, Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa, The region of spU2AF(59) that interacts with spU2AF(23) is similar to the region in which the human small and large subunits interact.
机译:人类剪接因子U2辅助因子(hsU2AF)由65和35 kDa的两个相互作用亚基组成,先前我们鉴定了人类大亚基的粟酒裂殖酵母同源物spU2AF(59),我们已经在中筛选了裂变酵母cDNA文库使用酵母双杂交系统搜索与spU2AF(59)相互作用并鉴定了hsU2AF(35)亚基的同源物的蛋白质,S.pombe U2AF小亚基是一个单拷贝基因,编码共享55%的蛋白质氨基酸同一性和与人类小亚基的17%相似性,与人类蛋白质不同,酵母蛋白质缺少富含精氨酸/丝氨酸的区域。 spU2AF小亚基的预测分子量为23 kDa。与spU2AF(23)相互作用的spU2AF(59)区域与人类小亚基和大亚基相互作用的区域相似。

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