...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic Acids Research >THE BASIC DOMAIN LEUCINE ZIPPER PROTEIN HXBP-1 PREFERENTIALLY BINDS TO AND TRANSACTIVATES CRE-LIKE SEQUENCES CONTAINING AN ACGT CORE
【24h】

THE BASIC DOMAIN LEUCINE ZIPPER PROTEIN HXBP-1 PREFERENTIALLY BINDS TO AND TRANSACTIVATES CRE-LIKE SEQUENCES CONTAINING AN ACGT CORE

机译：基本域白蛋白拉链蛋白HXBP-1优先结合并交易包含ACGT核的类致病序列

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The transcription factor hXBP-1 belongs to the family of basic region/leucine zipper (bZIP) proteins and interacts with the cAMP responsive element (CRE) of the major histocompatibility complex (MHC) class II A alpha, DR alpha and DP beta genes. However, the developmental expression of hXBP-1 as revealed by in situ hybridization in mouse embryos, has suggested that it interacts with the promoter of additional genes, To identify other potential target genes of this factor, we performed binding site selection experiments with recombinant hXBP-1 protein, The results indicated that hXBP-1 binds preferably to the CRE-like element GAT-GACGTG(T/G)NNN(A/T)T, wherein the core sequence ACGT is highly conserved, and that it also binds to some TPA response elements (TRE), hXBP-1 can transactivate multimers of the target sequences to which it binds in COS cells, and the level of transactivation directly correlates with the extent of binding as observed in gel retardation experiments, One target sequence that is strongly bound by hXBP-1 is the 21 bp repeat in the HTLV-1 LTR, and we demonstrate here that hXBP-1 can transactivate the HTLV-I LTR, Further, the transactivation domain of hXBP-1 encompasses a large C-terminal region of the protein, containing domains rich in glutamine, serine and threonine, and proline and glutamine residues, as shown in transient transfection experiments using hXBP-1-GAL4 fusion proteins and a reporter gene under the control of GAL4-binding sites.
机译：转录因子hXBP-1属于碱性区域/亮氨酸拉链（bZIP）蛋白家族，并与主要组织相容性复合体（MHC）II类Aα，DRα和DPβ基因的cAMP响应元件（CRE）相互作用。然而，通过在小鼠胚胎中原位杂交揭示了hXBP-1的发育表达，表明它与其他基因的启动子相互作用。为鉴定该因子的其他潜在靶基因，我们用重组hXBP进行了结合位点选择实验-1蛋白，结果表明hXBP-1优选结合CRE样元件GAT-GACGTG（T / G）NNN（A / T）T，其中核心序列ACGT是高度保守的，并且它还结合一些TPA反应元件（TRE），hXBP-1可以在COS细胞中激活与其结合的靶序列的多聚体，并且如在凝胶阻滞实验中观察到的那样，激活的水平与结合程度直接相关。被hXBP-1强烈结合的是HTLV-1 LTR中的21 bp重复序列，我们在这里证明了hXBP-1可以反式激活HTLV-1 LTR。此外，hXBP-1的反式激活结构域包含一个大的C端区域蛋白质含有丰富的谷氨酰胺，丝氨酸和苏氨酸以及脯氨酸和谷氨酰胺残基的结构域，如使用hXBP-1-GAL4融合蛋白和报道基因在GAL4结合位点控制下的瞬时转染实验所示。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号