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首页> 外文期刊>Nucleic Acids Research >MITOCHONDRIAL DNA POLYMERASE GAMMA IS EXPRESSED AND TRANSLATED IN THE ABSENCE OF MITOCHONDRIAL DNA MAINTENANCE AND REPLICATION
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MITOCHONDRIAL DNA POLYMERASE GAMMA IS EXPRESSED AND TRANSLATED IN THE ABSENCE OF MITOCHONDRIAL DNA MAINTENANCE AND REPLICATION

机译：线粒体DNA聚合酶γ在线粒体DNA缺乏维持和复制的情况下表达和转化。

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摘要

Mitochondria are essential organelles in all eukaryotic cells where cellular ATP is generated through the process of oxidative phosphorylation. Protein components of the respiratory assembly are gene products of both mitochondrial and nuclear genes, The mitochondrial genome itself encodes several protein and nucleic acid components required for such oxidative phosphorylative processes, but the vast majority of genes encoding respiratory chain components are nuclear, Similarly, the processes of replication and transcription of mitochondrial DNA rely exclusively upon RNA and protein species encoded by nuclear genes, We have analyzed two key nuclear-encoded proteins involved in mitochondrial DNA replication and transcription as a function of the presence or absence of mitochondrial DNA, Mitochondrial DNA polymerase (DNA polymerase gamma), the nuclear-encoded enzyme which synthesizes mtDNA, is expressed and translated in cells devoid of mitochondrial DNA itself, In contrast, mitochondrial transcription factor A protein levels are tightly linked to the mtDNA status of the cell, These results demonstrate that the DNA polymerase gamma protein is stable in the absence of mitochondrial DNA, and that there appears to be no regulatory mechanism present in these cells to alter levels of this protein in the complete absence of mitochondrial DNA, Alternatively it is possible that this enzyme plays an additional, as yet undefined, role in the cell, thereby mandating its continued production.
机译：线粒体是所有真核细胞中必不可少的细胞器，在这些真核细胞中，细胞ATP通过氧化磷酸化过程产生。呼吸道装配的蛋白质成分是线粒体和核基因的基因产物。线粒体基因组本身编码这种氧化磷酸化过程所需的几种蛋白质和核酸成分，但是编码呼吸链成分的绝大多数基因都是核的。线粒体DNA的复制和转录过程仅依赖于RNA和核基因编码的蛋白质种类。我们分析了与线粒体DNA的存在或不存在有关的线粒体DNA线粒体DNA的存在与否的函数，分析了两种关键的核编码蛋白聚合酶（DNA polymerase gamma）是合成mtDNA的核编码酶，在没有线粒体DNA本身的细胞中表达和翻译。相反，线粒体转录因子A的蛋白水平与细胞的mtDNA状态紧密相关，这些结果证明DNA聚合酶在没有线粒体DNA的情况下，蛋白质是稳定的，而在完全不存在线粒体DNA的情况下，这些细胞中似乎没有调节机制来改变该蛋白质的水平。尚未确定的角色在细胞中的作用，从而要求其继续生产。

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