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首页> 外文期刊>Nucleic Acids Research >APURINIC/APYRIMIDINIC (AP) ENDONUCLEASE FROM DICTYOSTELIUM DISCOIDEUM - CLONING, NUCLEOTIDE SEQUENCE AND INDUCTION BY SUBLETHAL LEVELS OF DNA DAMAGING AGENTS
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APURINIC/APYRIMIDINIC (AP) ENDONUCLEASE FROM DICTYOSTELIUM DISCOIDEUM - CLONING, NUCLEOTIDE SEQUENCE AND INDUCTION BY SUBLETHAL LEVELS OF DNA DAMAGING AGENTS

机译：迪斯科双歧杆菌的APURINIC / APRYRIMIDINIC（AP）核糖核酸酶-克隆，核苷酸序列和DNA破坏剂的潜在水平诱导

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摘要

We have cloned an AP endonuclease gene (APEA) from Dictyostelium discoideum, along with 1.8 kb of the 5' flanking region, There are no introns. The sequence predicts a protein of 361 amino acids, showing high homology to the major human/Escherichia coli exonuclease III family of AP endonucleases. There is 47% identity and 64% similarity to the Ape endonuclease of human cells using the C-terminal 257 amino acids of the Dictyostelium protein. The 104 amino acids on the N-terminus shaw only low homology with other AP endonucleases. Instead, this region shows high homology with the acid-rich regions of proteins associated with chromatin, such as nucleolins and HMG proteins. The gene is transcriptionally activated up to 7-fold after treatment of cells with sublethal levels of DNA damaging agents, including ultraviolet light, MNNG and bleomycin. Induction does not occur following blocking of replication fork polymerases with aphidicolin. It is not eliminated by treatment with kinase or phosphatase inhibitors. Four DNA damage-sensitive mutants all retained the DNA damage-induced up-regulation.
机译：我们已经从Disctyostelium discoideum克隆了一个AP核酸内切酶基因（APEA），以及1.8 kb的5'侧翼区域，没有内含子。该序列预测一种361个氨基酸的蛋白质，与主要的人类/大肠杆菌外切核酸酶III家族的AP核酸内切酶具有高度同源性。使用Dictyostelium蛋白的C端257个氨基酸，与人类细胞的Ape核酸内切酶有47％的同源性和64％的相似性。 N末端的104个氨基酸与其他AP核酸内切酶的同源性很低。相反，该区域与与染色质相关的蛋白质（如核仁蛋白和HMG蛋白质）的富酸区域表现出高度同源性。在用亚致死水平的DNA破坏剂（包括紫外线，MNNG和博来霉素）处理细胞后，该基因被转录激活多达7倍。用叉环二磷酸阻断复制叉聚合酶后不会发生诱导。不能通过激酶或磷酸酶抑制剂的治疗消除。四个对DNA损伤敏感的突变体均保留了DNA损伤诱导的上调。

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