S-1 NUCLEASE HYBRID ANALYSIS OF MITOCHONDRIAL DNA AMPLIFIED BY LONG-DISTANCE PCR - RAPID SCREENING FOR SMALL-SCALE REARRANGEMENTS

Lundin K. Wilichowski E. Ernst BP. Hanefeld F.

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S-1 NUCLEASE HYBRID ANALYSIS OF MITOCHONDRIAL DNA AMPLIFIED BY LONG-DISTANCE PCR - RAPID SCREENING FOR SMALL-SCALE REARRANGEMENTS

机译：长距离PCR扩增的线粒体DNA S-1核酸杂交分析-快速筛选小范围的重组

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We report on a method suitable for screening large regions (>3 kb) of mtDNA for structural changes of <500 bp and their localization. Heteroduplexes consisting of a wild-type and a mutant strand are cleaved by S-1 nuclease when single-stranded loops are present due to deletions or duplications/insertions. This strategy was successfully applied to screen the muscle mtDNA of 20 patients with mitochondrial encephalomyopathies. In three of them, an altered cleavage pattern was observed caused by a homoplasmic 9 bp deletion as shown by subsequent mapping and sequencing studies.

机译：我们报告了一种适合筛选mtDNA大区域（> 3 kb）的方法，用于<500 bp的结构变化及其定位。当由于缺失或重复/插入而存在单链环时，由S-1核酸酶切割由野生型和突变链组成的异源双链体。该策略已成功应用于筛查20例线粒体脑脊髓病患者的肌肉mtDNA。在其中的三个中，观察到了由同质9 bp缺失引起的切割模式改变，如随后的作图和测序研究所示。

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《Nucleic Acids Research》 |1997年第12期|共2页
作者
Lundin K. Wilichowski E. Ernst BP. Hanefeld F.;
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正文语种 eng
中图分类细胞形态学;生物化学;
关键词
Amplification; Deletion; Muscle;

机译：放大;删除;肌肉;

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1. S-1 NUCLEASE HYBRID ANALYSIS OF MITOCHONDRIAL DNA AMPLIFIED BY LONG-DISTANCE PCR - RAPID SCREENING FOR SMALL-SCALE REARRANGEMENTS [J] . Lundin K. Wilichowski E. Ernst BP. Hanefeld F. Nucleic Acids Research . 1997,第12期

机译：长距离PCR扩增的线粒体DNA S-1核酸杂交分析-快速筛选小范围的重组
2. Erratum to: A rapid and reliable method for PCR-based amplification of chromosomal and mitochondrial DNA from intact yeast cells [J] . R. Jesenofsky, J. Naehring, K. Wolf Current Genetics . 2011,第6期

机译：勘误到：一种快速可靠的方法，用于基于PCR的完整酵母细胞中染色体和线粒体DNA的扩增
3. The use of duplex-specific crab nuclease for rapid analysis of single-nucleotide polymorphisms and the detection of DNA targets in complex PCR products [J] . Shagina I., Bogdanova E., Altshuler I., Russian journal of bioorganic chemistry . 2011,第4期

机译：使用双链体特异性螃蟹核酸酶快速分析单核苷酸多态性和检测复杂PCR产物中的DNA靶标
4. PCR Amplified Dnazyme-Amplicons for Generic Solid-Phase Antimicrobial Resistence Screening [C] . Bernd Peeters, Saba Safdar, Bram Carlier, International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII . 2019

机译：PCR扩增的Dnazyme-Amplicons，用于常规固相抗微生物筛选
5. PCR amplification and sequencing of mitochondrial DNA from the rotifer Brachionus plicatilis. [D] . Li, Jia Jenny. 1997

机译：轮虫Brachionus plicatilis线粒体DNA的PCR扩增和测序。
6. S1 nuclease hybrid analysis of mitochondrial DNA amplified by long-distance PCR: rapid screening for small-scale rearrangements. [O] . K Lundin, E Wilichowski, B P Ernst, 1997

机译：通过长距离PCR扩增的线粒体DNA的S1核酸酶杂交分析：小规模重排的快速筛选。
7. S1 nuclease hybrid analysis of mitochondrial DNA amplified by long-distance PCR: rapid screening for small-scale rearrangements. [O] . Lundin, K, Wilichowski, E, Ernst, B P, 1997

机译：通过长距离PCR扩增的线粒体DNA的S1核酸酶杂交分析：小规模重排的快速筛选。

S-1 NUCLEASE HYBRID ANALYSIS OF MITOCHONDRIAL DNA AMPLIFIED BY LONG-DISTANCE PCR - RAPID SCREENING FOR SMALL-SCALE REARRANGEMENTS

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