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首页> 外文期刊>Nucleic Acids Research >VARIATIONS OF THE C2H2 ZINC FINGER MOTIF IN THE YEAST GENOME AND CLASSIFICATION OF YEAST ZINC FINGER PROTEINS
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VARIATIONS OF THE C2H2 ZINC FINGER MOTIF IN THE YEAST GENOME AND CLASSIFICATION OF YEAST ZINC FINGER PROTEINS

机译：酵母基因组中C2H2锌指基态的变化及酵母锌指蛋白的分类

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摘要

The PROSITE pattern Zinc_Finger_C2H2 was extended to permit the detection of all C2H2 zinc fingers and their parent proteins in the recently completed sequence of the yeast genome. Additionally, a new computer program was written that extracts other zinc binding motifs (non C2H2 'fingers'), overlapping with the classical zinc finger pattern, from the found set of yeast C2H2 fingers. The complete and correct detection of all fingers is a prerequisite for the classification of the yeast zinc finger proteins in functional terms. The detected 53 yeast C2H2 zinc finger proteins do not contain finger clusters with 10 or more repeats, as is frequently found in higher eukaryotes. Only three proteins contain four or more fingers in a cluster. Moreover, nearly all 27 yeast proteins with tandem arrays of two or three finger domains can be classified into nine subgroups with high sequence conservation in their finger clusters, in particular of their DNA recognition helices. These results and application of the recently elaborated finger/DNA recognition rules suggest that the yeast proteins belonging to the same subgroup may recognize identical or very similar DNA sites.
机译：扩展了PROSITE模式Zinc_Finger_C2H2以允许在酵母基因组的最新完成序列中检测所有C2H2锌指及其亲本蛋白。此外，编写了一个新的计算机程序，从发现的酵母C2H2手指集中提取了与经典锌指图案重叠的其他锌结合基序（非C2H2“手指”）。完整正确地检测所有手指是按功能术语对酵母锌指蛋白进行分类的先决条件。正如在高等真核生物中经常发现的那样，检测到的53种酵母C2H2锌指蛋白不包含具有10个或10个以上重复序列的指簇。只有三个蛋白质在一个簇中包含四个或更多个手指。此外，几乎所有具有两个或三个手指结构域串联排列的27种酵母蛋白质都可以分为九个亚组，这些亚组在其手指簇中具有高度的序列保守性，尤其是在其DNA识别螺旋中。这些结果和最近拟定的手指/ DNA识别规则的应用表明，属于同一亚组的酵母蛋白可以识别相同或非常相似的DNA位点。

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