...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic Acids Research >IDENTIFICATION OF PROTEINS BINDING SPECIFICALLY TO THE 3'-UNTRANSLATED REGION OF GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR MRNA
【24h】

IDENTIFICATION OF PROTEINS BINDING SPECIFICALLY TO THE 3'-UNTRANSLATED REGION OF GRANULOCYTE MACROPHAGE-COLONY STIMULATING FACTOR MRNA

机译：特定结合到粒细胞巨噬细胞-结肠刺激因子mRNA的3'-未翻译区的蛋白的鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 3'-untranslated region of granulocyte/macrophage colony-stimulating factor (GM-CSF) mRNA contributes to the post-transcriptional regulation of gene expression. Degradation is partly mediated by adenosine-uridine-rich sequence elements (ARE), which serve as binding sites for specific proteins. Stabilization of RNA by phytohemagglutinin and concanavalin A treatment is dependent on regulatory sequence elements upstream of ARE. We have performed northwestern blot and filter binding assays using cell extracts and RNA sequences containing or lacking ARE. Murine and human T cell extracts (EL-4 and Jurkat) yielded two specific proteins of 93 and 94 kDa, respectively, that were binding to sequences upstream of ARE. Within this region, the human and murine RNA do not share any obvious sequence identity, yet both are target sites for the binding proteins. The smallest RNA fragments protected by the proteins from RNase A digestion, were 44 in the murine, and 38 ribonucleotides long in the human sequence. The binding activity of the 94 kDa protein derived from human Jurkat cells could be enhanced by phytohemagglutinin. The interaction with regulatory mRNA sequences and the responsiveness to phytohemagglutinin suggests that the proteins are involved in controlling GM-CSF mRNA turnover.
机译：粒细胞/巨噬细胞集落刺激因子（GM-CSF）mRNA的3'非翻译区有助于基因表达的转录后调控。降解部分地由富含腺苷-尿苷的序列元件（ARE）介导，所述序列元件充当特定蛋白质的结合位点。植物血凝素和伴刀豆球蛋白A对RNA的稳定作用取决于ARE上游的调控序列元件。我们已经使用细胞提取物和含有或缺乏ARE的RNA序列进行了西北印迹和滤膜结合测定。鼠和人T细胞提取物（EL-4和Jurkat）分别产生93和94 kDa的两个特定蛋白，这些蛋白与ARE上游的序列结合。在该区域内，人类和鼠类RNA没有任何明显的序列同一性，但两者都是结合蛋白的靶位点。受RNase A消化的蛋白质保护的最小RNA片段在鼠中为44条，在人序列中长为38个核糖核苷酸。植物血凝素可增强源自人Jurkat细胞的94 kDa蛋白的结合活性。与调节性mRNA序列的相互作用和对植物血凝素的响应性表明，这些蛋白参与了控制GM-CSF mRNA的转换。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号