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首页> 外文期刊>Nucleus >The inner nuclear membrane proteins Mani and lmai link to two different types of chromatin at the nuclear periphery in S. pombe
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The inner nuclear membrane proteins Mani and lmai link to two different types of chromatin at the nuclear periphery in S. pombe

机译：内核膜蛋白Mani和lmai在粟酒裂殖酵母核外围与两种不同类型的染色质连接

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Metazoan chromatin at the nuclear periphery is generally characterized by lowly expressed genes and repressive chromatin marks and presents a sub-compartment with properties distinct from the nuclear interior. To test whether the S. pombe nuclear periphery behaves similarly, we used DNA adenine methyltransferase identification (DamID) to map the target loci of two inner nuclear membrane proteins, Ima1 and Man1. We found that peripheral chromatin shows low levels of RNA-Polymerase II and nucleosome occupancy, both characteristic of repressed chromatin regions. Consistently, lowly expressed genes preferentially associate with the periphery and highly expressed genes are depleted from it. When looking at peripheral intergenic regions (IGRs), we found that divergent IGRs are enriched compared with convergent IGRs, indicating that transcription preferentially points away from the periphery rather than toward it. Interestingly, we found that Ima1 and Mani have common, but also separate target regions in the genome. Ima1-interacting loci were enriched for the RNAi components Dcr1 and Rdp1. This agrees with previous findings that Dcr1 is localized at the nuclear periphery. In contrast, Mani target loci were bound by the heterochromatin protein Swi6, especially at subtelomeric regions. Subtelomeric chromatin was shown to form a unique chromatin type lacking both repressive and active chromatin features and containing low levels of the histone variant H2A.Z. Thus, we find that the fission yeast nuclear periphery shows similar properties to those of metazoan cells, despite the absence of a nuclear lamina. Our results point to a role of nuclear membrane proteins in organizing chromatin domains and loops.
机译：核外围的后生染色质通常以低表达的基因和抑制性染色质标记为特征，并呈现出具有不同于核内部特性的小室。为了测试粟酒裂殖酵母核外围的行为是否类似，我们使用DNA腺嘌呤甲基转移酶鉴定（DamID）绘制了两个内部核膜蛋白Ima1和Man1的靶基因座。我们发现，外周染色质显示出低水平的RNA聚合酶II和核小体占有率，这两个都是染色质区域受阻的特征。一致地，低表达的基因优先与外周结合，而高表达的基因则从外周消除。当查看外围基因间区域（IGR）时，我们发现与聚合IGR相比，发散的IGR更加丰富，这表明转录优先指向外围而不是外围。有趣的是，我们发现Ima1和Mani在基因组中具有共同但又分开的目标区域。与Ima1相互作用的基因座富含RNAi成分Dcr1和Rdp1。这与以前的发现一致，即Dcr1位于核外围。相反，Mani目标基因座被异染色质蛋白Swi6结合，特别是在亚端粒区域。显示出亚端粒染色质形成独特的染色质类型，既缺乏抑制性又没有活性的染色质特征，并且含有低水平的组蛋白变体H2A.Z。因此，我们发现裂变酵母的核外围显示出与后生细胞相似的特性，尽管没有核层。我们的结果指出核膜蛋白在组织染色质结构域和环中的作用。

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