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首页> 外文期刊>Nucleus >‘Natively unfolded’ nucleoporins in nucleocytoplasmic transport Clustered or evenly distributed?
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‘Natively unfolded’ nucleoporins in nucleocytoplasmic transport Clustered or evenly distributed?

机译：核质运输中的“天然展开”的核孔蛋白成簇还是均匀分布？

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摘要

The nuclear pore complex (NPC) acts as a selective gate that mediates the bidirectional transport of macromolecules between the cytoplasm and the nucleus of eukaryotic cells. ‘Natively unfolded’ nucleoporins (Nups) with domains rich in phenylalanine-glycine (FG) repeats form the selective permeability barrier and provide binding sites for mobile transport receptors in the NPC. Understanding the structure and function of the FG-Nups barrier under real-time trafficking conditions is still a formidable challenge due to the dynamic nature of a channeled membranous environment. Recently, we have shown that three-dimensional (3D) density maps of transient interactions between the FG-Nups barrier and a cargo-free or a cargo-bound transport receptor in native NPCs can be obtained by an advanced single-molecule fluorescence microscopy approach. Moreover, we found that these interaction sites are spatially clustered into distinct groups in the periphery around a central axial channel with a diameter of approximately 10–20 nm in the NPC. The 3D distribution of interaction sites may indicate some native properties of the FG-Nups barrier. Here we speculate that the selective permeability barrier in the NPC could be formed by clustered FG-Nups.
机译：核孔复合物（NPC）充当选择性门，介导大分子在真核细胞胞质和细胞核之间的双向运输。结构域富含苯丙氨酸-甘氨酸（FG）重复序列的“天然折叠”核孔蛋白（Nups）形成了选择性渗透屏障，并为NPC中的移动转运受体提供了结合位点。由于通道膜环境的动态性质，在实时贩运条件下了解FG-Nups屏障的结构和功能仍然是一个艰巨的挑战。最近，我们已经表明，可以通过先进的单分子荧光显微镜方法获得FG-Nups屏障与天然NPC中无货物或与货物结合的运输受体之间的瞬时相互作用的三维（3D）密度图。此外，我们发现这些相互作用的位点在NPC中围绕中心轴向通道周围的空间在空间上分为不同的组，中心轴向通道的直径约为10-20 nm。相互作用位点的3D分布可能表明FG-Nups屏障的某些天然属性。在这里，我们推测，NPC中的选择性渗透性屏障可以由成簇的FG-Nups形成。

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