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SUN proteins and nuclear envelope spacing

机译:SUN蛋白与核包膜间距

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The nuclear envelope consists of 2 membranes separated by 30-50nm, but how the 2 membranes are evenly spaced has been an open question in the field. Nuclear envelope bridges composed of inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins have been proposed to set and regulate nuclear envelope spacing. We tested this hypothesis directly by examining nuclear envelope spacing in Caenorhabditis elegans animals lacking UNC-84, the sole somatic SUN protein. SUN/KASH bridges are not required to maintain even nuclear envelope spacing in most tissues. However, UNC-84 is required for even spacing in body wall muscle nuclei. Shortening UNC-84 by 300 amino acids did not narrow the nuclear envelope space. While SUN proteins may play a role in maintaining nuclear envelope spacing in cells experiencing forces, our data suggest they are dispensable in most cells.
机译:核膜由2个间隔30-50nm的膜组成,但是如何均匀地隔开2个膜一直是该领域的一个悬而未决的问题。已经提出了由内核膜SUN蛋白和外核膜KASH蛋白组成的核包膜桥来设定和调节核包膜间隔。我们通过检查缺乏唯一的体细胞SUN蛋白UNC-84的秀丽隐杆线虫动物的核被膜间距来直接检验该假设。不需要SUN / KASH桥即可在大多数组织中保持均匀的核包膜间距。但是,UNC-84是在体壁肌肉核中均匀分布所必需的。 UNC-84缩短300个氨基酸不会使核被膜空间变窄。虽然SUN蛋白可能在维持受力细胞中维持核被膜间距中起作用,但我们的数据表明它们在大多数细胞中都是不可缺少的。

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