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首页> 外文期刊>Cell metabolism >Incompatibility between Nuclear and Mitochondrial Genomes Contributes to an Interspecies Reproductive Barrier
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Incompatibility between Nuclear and Mitochondrial Genomes Contributes to an Interspecies Reproductive Barrier

机译：核和线粒体基因组之间的不相容性导致种间繁殖障碍。

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Vertebrate cells carry two different genomes, nuclear (nDNA) and mitochondrial (mtDNA), both encoding proteins involved in oxidative phosphorylation. Because of the extensive interactions, adaptive coevolution of the two genomes must occur to ensure normal mitochondrial function. To investigate whether incompatibilities between these two genomes could contribute to interspecies reproductive barriers, we performed reciprocal mtDNA replacement (MR) in zygotes between widely divergent Mus m. domesticus (B6) and conplastic Mus m. musculus (PWD) mice. Transfer of MR1 cybrid embryos (B6nDNA-PWDmtDNA) supported normal development of F1 offspring with reduced male fertility but unaffected reproductive fitness in females. Furthermore, donor PWD mtDNA was faithfully transmitted through the germline into F2 and F3 generations. In contrast, reciprocal MR2 (PWDnDNA-B6mtDNA) produced high embryonic loss and stillborn rates, suggesting an association between mitochondrial function and infertility. These results strongly suggest that functional incompatibility between nuclear and mitochondrial genomes contributes to interspecies reproductive isolation in mammals.
机译：脊椎动物细胞带有两个不同的基因组，核（nDNA）和线粒体（mtDNA），它们都编码参与氧化磷酸化的蛋白质。由于广泛的相互作用，必须发生两个基因组的自适应协同进化，以确保正常的线粒体功能。为了研究这两个基因组之间的不相容性是否可能导致种间繁殖障碍，我们在彼此不同的Mus m之间的受精卵中进行了相互的mtDNA置换（MR）。家畜（B6）和结实小鼠。小家鼠（PWD）小鼠。 MR1杂种胚胎（B6nDNA-PWDmtDNA）的转移支持F1子代的正常发育，降低了男性的生育能力，但女性的生殖健康却未受影响。此外，供体PWD mtDNA忠实地通过种系传播到F2和F3代。相反，相互的MR2（PWDnDNA-B6mtDNA）产生高的胚胎损失和死胎率，表明线粒体功能与不育之间存在关联。这些结果强烈表明，核基因组和线粒体基因组之间的功能不兼容是哺乳动物间种间生殖分离的原因。

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