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首页> 外文期刊>Cell motility and the cytoskeleton >Monastral bipolar spindles in meiosis II of male Trichosia pubescens (Sciaridae): early stages of spindle formation and chromosome orientation.
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Monastral bipolar spindles in meiosis II of male Trichosia pubescens (Sciaridae): early stages of spindle formation and chromosome orientation.

机译：雄性Trichosia pubescens（Sciaridae）减数分裂II中的单亲双极纺锤体：纺锤体形成和染色体方向的早期阶段。

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摘要

The metaphase spindle of male meiosis II in fungus gnats (Sciaridae) is one example of naturally occurring monastral bipolar spindles. To gain further insights into how the bipolar spindle is formed in the presence of only one polar center, prometaphase of male meiosis II was investigated in the sciarid Trichosia pubescens by means of anti-tubulin immunofluorescence, DAPI chromosome staining, and electron microscopy of ultrathin serial sections. The first step in spindle formation after interkinesis seems to be the organization of an astral half-spindle, probably by MTOC activity of the astral region. With the exception of the non-disjunctional X chromosome, which always lies close to the aster, the chromosomes are found to occupy various positions with respect to the astral region, revealing different orientations of their chromatid kinetochores. It was observed that some of the mal-oriented kinetochores are associated with microtubules that, due to their orientation perpendicular to the spindle axis, are unlikely to originate from the astral region. Therefore, these mal-oriented microtubules are taken as an indication of a dispersed MTOC activity near the chromosomes or at kinetochores. According to recent models of chromosome-induced spindle self-organization [e.g., Merdes et al., 1997: J. Cell Biol. 138:953-956], they could be responsible for the formation of the other (anastral) half-spindle and for amphitelic (bipolar) orientation of the chromosomes. Copyright 1999 Wiley-Liss, Inc.
机译：真菌（Sciaridae）中雄性减数分裂II的中期纺锤体是自然发生的单峰双极纺锤体的一个例子。为了进一步了解仅存在一个极性中心的情况下双极纺锤体是如何形成的，通过抗微管蛋白免疫荧光，DAPI染色体染色和超薄系列电子显微镜观察了男性坐骨神经减数分裂II的前期。部分。跨动后纺锤体形成的第一步似乎是一个星形半纺锤体的组织，可能是通过纺锤体区域的MTOC活性来实现的。除了始终位于紫苑附近的非分离X染色体外，发现这些染色体相对于星体区域占据了不同的位置，从而揭示了其染色单体着体的不同方向。观察到，一些恶意取向的动植物与微管有关，由于它们垂直于纺锤轴，因此微管不太可能源自星体区域。因此，这些不良取向的微管被认为是在染色体附近或在动植物处分散的MTOC活性的指示。根据染色体诱导的纺锤体自组织的最新模型[例如，Merdes等人，1997：J.Cell Biol.Chem.Soc。，66，1897]。 138：953-956]，它们可能负责形成另一条（肛门）半纺锤体，并导致染色体两性（双极）取向。版权所有1999 Wiley-Liss，Inc.

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