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首页> 外文期刊>Cell motility and the cytoskeleton >NuMA is a component of an insoluble matrix at mitotic spindle poles.
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NuMA is a component of an insoluble matrix at mitotic spindle poles.

机译：NuMA是有丝分裂纺锤体极的不溶性基质的成分。

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摘要

NuMA associates with microtubule motors during mitosis to perform an essential role in organizing microtubule minus ends at spindle poles. Using immunogold electron microscopy, we show that NuMA is a component of an electron-dense material concentrated at both mitotic spindle poles in PtK1 cells and the core of microtubule asters formed through a centrosome-independent mechanism in cell-free mitotic extracts. This NuMA-containing material is distinct from the peri-centriolar material and forms a matrix that appears to anchor microtubule ends at the spindle pole. In stark contrast to conventional microtubule-associated proteins whose solubility is directly dependent on microtubules, we find that once NuMA is incorporated into this matrix either in vivo or in vitro, it becomes insoluble and this insolubility is no longer dependent on microtubules. NuMA is essential for the formation of this insoluble matrix at the core of mitotic asters assembled in vitro because the matrix is absent from mitotic asters assembled in a cell-free mitotic extract that is specifically depleted of NuMA. These physical properties are consistent with NuMA being a component of the putative mitotic spindle matrix in vertebrate cells. Furthermore, given that NuMA is essential for spindle pole organization in vertebrate systems, it is likely that this insoluble matrix plays an essential structural function in anchoring and/or stabilizing microtubule minus ends at spindle poles in mitotic cells.
机译：在有丝分裂期间，NuMA与微管运动相关联，在组织微管负端的纺锤体轴端中发挥重要作用。使用免疫金电子显微镜，我们显示NuMA是电子致密材料的一个组成部分，该材料集中在PtK1细胞的两个有丝分裂纺锤体极点和通过无细胞有丝分裂独立机制在无细胞有丝分裂提取物中形成的微管紫苑的核心。这种含NuMA的材料与中心小周周围的材料不同，并形成了将微管末端锚定在纺锤极的基质。与溶解度直接取决于微管的常规微管相关蛋白形成鲜明对比的是，我们发现，一旦将NuMA体内或体外掺入该基质中，它就会变得不溶，并且这种不溶性不再取决于微管。 NuMA对于在体外组装的有丝分裂紫苑的核心形成这种不可溶的基质至关重要，因为在无细胞的有丝分裂提取物中组装的有丝分裂紫苑中不存在该基质，而该有丝分裂的紫杉提取物特别消耗了NuMA。这些物理性质与NuMA是脊椎动物细胞中假定的有丝分裂纺锤体基质的成分一致。此外，鉴于NuMA对于脊椎动物系统中纺锤体的组织必不可少，这种不溶性基质可能在锚定和/或稳定有丝分裂细胞纺锤体的纺锤体负端中起着必不可少的结构功能。

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