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首页> 外文期刊>Cell motility and the cytoskeleton >Phosphorylation by CDK1 regulates XMAP215 function in vitro.
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Phosphorylation by CDK1 regulates XMAP215 function in vitro.

机译：CDK1的磷酸化在体外调节XMAP215功能。

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摘要

XMAP215, a microtubule-associated protein isolated from Xenopus eggs, promotes microtubule assembly dynamics in an end-specific manner: addition of XMAP215 to purified porcine tubulin increases both elongation and shortening rates at microtubule plus ends, with minimal effects at minus ends. Previous results indicated that XMAP215 is phosphorylated during M phase, suggesting that its activity may be regulated by cell cycle phosphorylation. To test this hypothesis, we used video-enhanced DIC microscopy to examine the effects of XMAP215 phosphorylated by CDK1 on the assembly of purified tubulin. XMAP215 incubated with ATP at 30 degrees C for 10- 20 min in the absence of CDK1 exhibited a 4.1-fold increase in plus end elongation rate compared to purified tubulin. Elongation was promoted to a lesser degree (2.4-fold) by phosphorylated XMAP215. In contrast, XMAP215 phosphorylation did not alter the approximately 3-fold increase in shortening rate. XMAP215 binding to taxol microtubules was also not changed by phosphorylation. To further investigate mechanisms responsible for the faster microtubule shortening rate observed with XMAP215, we made microtubules with segments assembled prior to XMAP215 addition (proximal segments) and segments assembled in the presence of XMAP215 (distal segments). In 9 of 10 microtubules, the distal segment shortened faster (distal = 60.7 microm/min; proximal = 37.5 microm/min), suggesting that MTs assembled in the presence of XMAP215 have an altered lattice that results in subsequent faster shortening. Copyright 1999 Wiley-Liss, Inc.
机译：XMAP215是一种从非洲爪蟾卵中分离的与微管相关的蛋白质，以末端特异性方式促进微管组装动力学：向纯化的猪微管蛋白中添加XMAP215既增加了微管正末端的伸长率，又缩短了其速率，负末端的影响最小。先前的结果表明XMAP215在M期被磷酸化，表明其活性可能受细胞周期磷酸化的调节。为了验证该假设，我们使用视频增强的DIC显微镜检查了CDK1磷酸化的XMAP215对纯化微管蛋白组装的影响。与纯化的微管蛋白相比，在不存在CDK1的情况下将XMAP215与ATP在30摄氏度下孵育10-20分钟，其正末端伸长率增加了4.1倍。磷酸化的XMAP215将伸长率提高到较小的程度（2.4倍）。相反，XMAP215磷酸化并没有改变缩短速率的大约3倍增加。 XMAP215与紫杉醇微管的结合也没有通过磷酸化而改变。为了进一步研究负责用XMAP215观察到更快的微管缩短率的机制，我们制作了具有在添加XMAP215之前组装的区段（近端区段）和在存在XMAP215的情况下组装的区段（远端区段）的微管。在10个微管中的9个中，远端节段缩短得更快（远端= 60.7 microm / min;近端= 37.5 microm / min），这表明在XMAP215存在下组装的MT具有改变的晶格，从而导致随后的更快缩短。版权所有1999 Wiley-Liss，Inc.

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