• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Neuronal intermediate filament protein alpha-internexin facilitates axonal neurite elongation in neuroblastoma cells.
【24h】

Neuronal intermediate filament protein alpha-internexin facilitates axonal neurite elongation in neuroblastoma cells.

机译：神经元中间丝蛋白α-internexin促进神经母细胞瘤细胞中的轴突神经突伸长。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined the localization and role of alpha-IN vs. other neuronal intermediate filaments before and during differentiation. Vimentin but not alpha-IN localized within filopodia-like neurites of undifferentiated cells. During differentiation, alpha-IN immunoreactivity accumulated within axonal neurites following vimentin but, as previously describe in neurons in situ, before the appearance of NF-L. We therefore manipulated alpha-IN synthesis, accumulation, and function in attempts to determine whether or not this intermediate filament species played a role in axonal development. Intracellular delivery of anti-alpha-IN antisense oligonucleotides and antibodies was permissive for neuritogenesis, yet compromised neurite elongation; this effect was further reflected in diminished levels of stabilized axonal microtubules. These data suggest that alpha-IN plays a role in the development of neuronal polarity. Relatively more alpha-IN than NF-L accumulated within the plastic axonal neurites induced following serum-deprivation, while stable, dbcAMP-induced neurites treatment contained equivalent levels of each. Protease inhibition increased NF-L and NF-H but not alpha-IN immunoreactivity within serum-deprived neurites, suggesting that proteolysis restricts NF-L accumulation pending neurite stabilization. To test the possibility that NF-H accumulation is dependent upon NF-L and cannot be mediated by alpha-IN, we examined levels of NF-H co-precipitated from cells with alpha-IN and NF-L. Virtually all newly synthesized NF-H co-precipitated with NF-L, while only a small percentage co-precipitated with alpha-IN. Finally, NF-H or NF-M were absent from the axon hillock or perikaryal area at the base of neurites, where alpha-IN immunoreactivity is prominent. These data extend earlier cell-free demonstrations that NF-H preferentially associates with NF-L rather than alpha-IN. Copyright 1999 Wiley-Liss, Inc.
机译：我们检查了分化之前和分化过程中α-IN与其他神经元中间丝的定位和作用。波形蛋白而不是α-IN定位在未分化细胞的丝状伪足样神经突内。在分化期间，波形蛋白之后，但是如先前在原位神经元中所述，在NF-L出现之前，α-IN免疫反应性积累在轴突神经突内。因此，我们操纵了α-IN的合成，积累和功能，试图确定这种中间丝在轴突发育中是否起作用。细胞内递送抗α-IN反义寡核苷酸和抗体可促进神经形成，但损害了神经突的延伸。这种作用在稳定的轴突微管水平降低中得到进一步体现。这些数据表明α-IN在神经元极性的发展中起作用。血清剥夺后诱导的可塑性轴突神经突中积累的NF-L相对比NF-L相对多，而稳定的dbcAMP诱导的神经突治疗包含相同的水平。蛋白酶抑制作用增加了血清剥夺的神经突中的NF-L和NF-H的表达，但没有增加α-IN的免疫反应性，表明蛋白水解作用限制了NF-L在神经突稳定之前的积累。为了测试NF-H积累依赖于NF-L且不能被α-IN介导的可能性，我们检查了与α-IN和NF-L共同沉淀的NF-H水平。几乎所有新合成的NF-H与NF-L共同沉淀，而只有一小部分与α-IN共同沉淀。最后，在轴突丘陵区或神经突底部的周围区域没有NF-H或NF-M，其中α-IN免疫反应性很明显。这些数据扩展了NF-H优先与NF-L而不是alpha-IN关联的早期无细胞演示。版权所有1999 Wiley-Liss，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号