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首页> 外文期刊>Cell motility and the cytoskeleton >An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning.
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An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning.

机译：莱茵衣藻的插入突变体，其微管定位有缺陷。

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摘要

cmu1-1 is a new mutation of Chlamydomonas reinhardtii that causes a change in cell shape due to an alteration of cytoplasmic microtubule organization. cmu1 mutant cells were first identified based on their altered cell shape. Unlike wild-type cells, which are ellipsoid, cmu1 cells tend to be either round or egg-shaped with the flagella extending from the narrow end of the cell. Electron microscopic comparison of mutant and wild-type cells indicated that microtubule distribution was altered in the mutant cells. Immunofluorescence microscopy using anti-beta-tubulin antibodies revealed that, in wild-type cells, microtubules arise from the anterior end of the cell in the region of the basal bodies, pass posteriorly subjacent to the plasma membrane, and terminate near the posterior end of the cell. In mutant cells, the microtubules also arise from the basal body region but then become disarrayed. They frequently curl back anteriorly or wrap around the equator of the cell; some microtubules also extend completely to the posterior end of the cell, then turn back toward the anterior end. No changes in the basal body region were detected by electron microscopy. Some cmu1 cells had multiple nuclei or an aberrant number of flagella, both of which may be due to defects in cell division, a process dependent upon microtubules. Thus, cmu1-1, which was generated by insertional mutagenesis and is tagged, appears to encode a protein that plays an essential role in the spatial organization of cytoplasmic microtubules involved in both interphase and mitotic functions. Copyright 1999 Wiley-Liss, Inc.
机译：cmu1-1是莱茵衣藻的新突变，由于细胞质微管组织的改变而引起细胞形状的改变。首先根据改变的细胞形状鉴定cmu1突变细胞。与椭圆形的野生型细胞不同，cmu1细胞倾向于为圆形或卵形，鞭毛从细胞的狭窄末端延伸。突变和野生型细胞的电子显微镜比较表明，突变细胞中微管的分布发生了变化。使用抗β微管蛋白抗体的免疫荧光显微镜检查显示，在野生型细胞中，微管起源于基体区域中细胞的前端，向后穿过质膜，并终止于质膜的后端附近。细胞。在突变细胞中，微管也来自基体区域，但随后变得混乱。它们经常向后卷曲或缠绕在细胞的赤道周围。一些微管也完全延伸到细胞的后端，然后向前端返回。通过电子显微镜未检测到基体区域的变化。一些cmu1细胞具有多个核或鞭毛数异常，这两者都可能是由于细胞分裂缺陷所致，该过程取决于微管。因此，由插入诱变产生并被标记的cmu1-1似乎编码一种蛋白质，该蛋白质在参与相间和有丝分裂功能的细胞质微管的空间组织中起着至关重要的作用。版权所有1999 Wiley-Liss，Inc.

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