...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Evidence for axonemal distortion during the flagellar beat of Chlamydomonas.
【24h】

Evidence for axonemal distortion during the flagellar beat of Chlamydomonas.

机译：衣藻鞭毛节拍期间轴突变形的证据。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to understand the working mechanism that governs the flagellar beat it is essential to know if the axoneme undergoes distortion during the course of the beat cycle. The rapid fixation method employed by Mitchell was able to preserve the waveform of Chlamydomonas flagella much as it appears during normal flagellar beating [Mitchell, Cell Motil Cytoskeleton 2003;56:120-129]. This conservation of the waveform suggests that the stress responsible for the production of bending is also trapped by the fixation procedure. Longitudinal sections of these well-preserved flagella were used to document variations in the relative axonemal diameter. Sections aligned to the plane of bending, showing both the central pair microtubules and outer doublets, were examined for this purpose. Micrographs were selected that continuously showed both the outer doublets and the central pair from a straight region to a curved region of the flagellum. Axoneme diameters measured from these select micrographs showed an increase in relative diameter that averaged 39 nm greater at the crest of the bent region. This constituted a 24% increase in the axoneme diameter in the bends. The transverse stress acting across the axoneme during bending was calculated from the Geometric Clutch computer model for a simulated Chlamydomonas-like flagellar beat. If we assume that this is representative of the transverse stress acting in a real flagellum, then the Young's modulus of the intact axoneme is approximately 0.02 MPa. The possibility that the distortion of the axoneme during the beat could play a significant role in regulating dynein function is discussed.
机译：为了了解控制鞭毛搏动的工作机制，必须知道轴突在搏动周期过程中是否发生变形。米切尔采用的快速固定方法能够保留衣藻的鞭毛波形，就像正常鞭毛殴打时一样[Mitchell，Cell Motil Cytoskeleton 2003; 56：120-129]。波形的这种守恒表明，固定过程也会捕获产生弯曲的应力。这些保存良好的鞭毛的纵切面用于记录相对轴突直径的变化。为此目的，检查了与弯曲平面对齐的截面，显示了中央对微管和外部双合体。选择显微照片，从鞭毛的笔直区域到弯曲区域连续显示外部对和中心对。从这些选择的显微照片测得的轴突直径显示出相对直径的增加，在弯曲区域的顶部平均增加了39 nm。这使弯头处的轴突直径增加了24％。从几何离合器计算机模型计算了弯曲过程中作用在轴突上的横向应力，用于模拟衣藻类鞭毛节拍。如果我们假设这代表了在实际鞭毛中作用的横向应力，则完整轴突的杨氏模量约为0.02 MPa。讨论了在搏动过程中轴蛋白变形可能在调节动力蛋白功能中起重要作用的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号