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首页> 外文期刊>Cell motility and the cytoskeleton >Jasplakinolide, an actin stabilizing agent, alters anaphase chromosome movements in crane-fly spermatocytes.
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Jasplakinolide, an actin stabilizing agent, alters anaphase chromosome movements in crane-fly spermatocytes.

机译：肌动蛋白稳定剂Jasplakinolide可改变鹤蝇精细胞的后期染色体运动。

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摘要

We added jasplakinolide to anaphase crane-fly spermatocytes and determined its effects on chromosome movement. Previous work showed that the actin depolymerizing agents cytochalasin D or latrunculin B blocked or slowed chromosome movements. We studied the effects of jasplakinolide, a compound that stabilizes actin filaments. Jasplakinolide had the same effect on movements of each half- bivalent in a separating pair of half-bivalents, but different half-bivalent pairs in the same cell often responded differently, even when the concentrations of jasplakinolide varied by a factor of two. Jasplakinolide had no effect on about 20% of the pairs, but otherwise caused movements to slow, or to stop, or, rarely, to accelerate. When cells were kept in jasplakinolide, stopped pairs eventually resumed movement; slowed pairs did not change their speeds. Confocal microscopy indicated that neither the distributions of spindle actin filaments nor the distributions of spindle microtubules were altered by the jasplakinolide. It is possible that jasplakinolide binds to spindle actin and blocks critical binding sites, but we suggest that jasplakinolide affects anaphase chromosome movement by preventing actin-filament depolymerization that is necessary for anaphase to proceed. Overall, our data indicate that actin is involved in one of the redundant mechanisms cells use to move chromosomes.
机译：我们将jasplakinolide添加到后期的鹤蝇精细胞中，并确定其对染色体运动的影响。先前的工作表明肌动蛋白解聚剂细胞松弛素D或拉特朗库林B阻止或减慢了染色体的移动。我们研究了jasplakinolide（一种稳定肌动蛋白丝的化合物）的作用。 Jasplakinolide对分开的半二价对中每个半二价的运动具有相同的影响，但是即使在Jasplakinolide的浓度变化两倍的情况下，同一细胞中不同的半二价对也常常具有不同的响应。 Jasplakinolide对约20％的成对没有影响，但以其他方式导致运动变慢，停止或很少加速。当细胞被保存在jasplakinolide中时，停止的配对最终恢复了运动。放慢脚步并没有改变他们的速度。共聚焦显微镜检查结果表明，茉莉酮醇内酯不改变纺锤体肌动蛋白丝的分布或纺锤体微管的分布。 jasplakinolide可能与纺锤状肌动蛋白结合并阻断关键的结合位点，但我们建议，jasplakinolide通过阻止后期进行的必要的肌动蛋白丝解聚作用，影响后期染色体的移动。总体而言，我们的数据表明肌动蛋白参与了细胞用于移动染色体的冗余机制之一。

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