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首页> 外文期刊>Cell motility and the cytoskeleton >The STE group kinase SepA controls cleavage furrow formation in Dictyostelium.
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The STE group kinase SepA controls cleavage furrow formation in Dictyostelium.

机译：STE组激酶SepA控制着Dictyostelium中切割沟的形成。

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摘要

During a REMI screen for proteins regulating cytokinesis in Dictyostelium discoideum we isolated a mutant forming multinucleate cells. The gene affected in this mutant encoded a kinase, SepA, which is an ortholog of Cdc7, a serine-threonine kinase essential for septum formation in Schizosaccharomyces pombe. Localization of SepA-GFP in live cells and its presence in isolated centrosomes indicated that SepA, like its upstream regulator Spg1, is associated with centrosomes. Knockout mutants of SepA showed a severe cytokinesis defect and a delay in development. In multinucleate SepA-null cells nuclear division proceeded normally and synchronously. However, often cleavage furrows were either missing or atypical: they were extremely asymmetric and constriction was impaired. Cortexillin-I, a marker localizing strictly to the furrow in wild-type cells, demonstrated that large, crescent-shaped furrows expanded and persisted long after the spindle regressed and nuclei returned to the interphase state. Outside the furrow the filamentous actin system of the cell cortex showed strong ruffling activity. These data suggest that SepA is involved in the spatial and temporal control system organizing cortical activities in mitotic and postmitotic cells.
机译：在REMI筛选过程中，我们在Discyostelium Discoideum中调节细胞分裂的蛋白质被分离出了一个突变体，形成了多核细胞。在该突变体中受影响的基因编码一个激酶SepA，它是Cdc7的直系同源物，Cdc7是一种在粟酒裂殖酵母中形成隔膜必不可少的丝氨酸-苏氨酸激酶。 SepA-GFP在活细胞中的定位及其在分离的中心体中的存在表明SepA与其上游调节剂Spg1一样，与中心体相关。 SepA的敲除突变体显示出严重的胞质分裂缺陷和发育延迟。在多核SepA-null细胞中，核分裂正常且同步进行。但是，开裂沟经常缺失或不典型：它们极不对称且收缩受到损害。 Cortexillin-I（一种严格定位于野生型细胞犁沟中的标记）表明，大的新月形犁沟在纺锤体退缩且细胞核返回中间状态后很长一段时间内一直扩张并持续存在。在沟的外面，细胞皮层的丝状肌动蛋白系统表现出很强的波纹作用。这些数据表明SepA参与组织有丝分裂和有丝分裂后细胞的皮质活动的空间和时间控制系统。

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