Mechanical dynamics of single cells during early apoptosis.

Pelling AE; Veraitch FS; Chu CP

首页> 外文期刊>Cell motility and the cytoskeleton >Mechanical dynamics of single cells during early apoptosis.

【24h】

Mechanical dynamics of single cells during early apoptosis.

机译：早期凋亡过程中单细胞的机械动力学。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Dynamic mechanical properties of cells are becoming recognized as indicators and regulators of physiological processes such as differentiation, malignant phenotypes and mitosis. A key process in development and homeostasis is apoptosis and whilst the molecular control over this pathway is well studied, little is known about the mechanical consequences of cell death. Here, we study the caspase-dependent mechanical kinetics of single cells during early apoptosis initiated with the general protein-kinase inhibitor staurosporine. This results in internal remodelling of the cytoskeleton and nucleus which is reflected in dynamic changes in the mechanical properties of the cell. Utilizing simultaneous confocal and atomic force microscopy (AFM), we measured distinct mechanical dynamics in the instantaneous cellular Young's Modulus and longer timescale viscous deformation. This allowed us to visualize time-dependent nuclear and cytoskeletal control of force dissipation with fluorescent fusion proteins throughout the cell. This work reveals that the cell death program not only orchestrates biochemical dynamics but also controls the mechanical breakdown of the cell. Importantly, the consequences of mechanical disregulation during apoptosis may be a contributing factor to several human pathologies through the poorly timed release of dead cells and cell debris.

机译：细胞的动态力学性能已被视为生理过程（例如分化，恶性表型和有丝分裂）的指示剂和调节剂。发育和体内平衡的关键过程是细胞凋亡，尽管对该途径的分子控制进行了深入研究，但对细胞死亡的机械后果知之甚少。在这里，我们研究了由普通蛋白激酶抑制剂星形孢菌素引发的早期凋亡过程中单细胞的半胱天冬酶依赖性机械动力学。这导致细胞骨架和细胞核的内部重塑，这反映在细胞机械性质的动态变化中。利用同时共焦和原子力显微镜（AFM），我们在瞬时细胞杨氏模量和较长时间尺度的黏性变形中测量了不同的机械动力学。这使我们能够可视化荧光融合蛋白在整个细胞中对力消散的时间依赖性核和细胞骨架控制。这项工作表明，细胞死亡程序不仅可以协调生物化学动力学，还可以控制细胞的机械故障。重要的是，凋亡过程中机械失调的后果可能是由于死细胞和细胞碎片释放不及时而导致的几种人类病理的因素。

著录项

来源
《Cell motility and the cytoskeleton》 |2009年第7期|共14页
作者
Pelling AE; Veraitch FS; Chu CP;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Mechanical dynamics of single cells during early apoptosis. [J] . Pelling AE, Veraitch FS, Chu CP Cell motility and the cytoskeleton . 2009,第7期

机译：早期凋亡过程中单细胞的机械动力学。
2. Dynamic mechanical measurement of the viscoelasticity of single adherent cells [J] . Elise A. Corbin, Olaoluwa O. Adeniba, Randy H. Ewoldt, Applied Physics Letters . 2016,第9期

机译：动态力学测量单个贴壁细胞的粘弹性
3. Hydrodynamic stretching of single cells for large population mechanical phenotyping [J] . Daniel R. Gossett, Henry T. K. Tse, Serena A. Lee, Proceedings of the National Academy of Sciences of the United States of America . 2012,第20期

机译：单细胞的流体动力学拉伸用于大量机械表型
4. Real-Time Three-Dimensional Single-Cell-Resolution Monitoring System for Observation of Dynamic Cell Behavior under Mechanical Stimuli [C] . Keitaro Kasahara, Yuta Kurashina, Shigenori Miura, IEEE International Conference on Micro Electro Mechanical Systems . 2021

机译：用于观察机械刺激下动态细胞行为的实时三维单细胞分辨率监测系统
5. Seeing the forest by looking at the trees: A single cell analysis of apoptosis. [D] . Goldstein, Joshua Carl Baba. 2001

机译：通过看树看森林：凋亡的单细胞分析。
6. Mechanical decision trees for investigating and modulating single-cell cancer invasion dynamics [O] . Michael Mak, David Erickson -1

机译：机械决策树用于研究和调节单细胞癌症侵袭动力学
7. P235Relation between myocardial deformation by three-dimensional speckle tracking analysis , control of the hypertension and functional capacity in patients with systemic hypertensionP236Obstructive sleep apnoea is often under-recognised in patients with acute myocardial infarction, but commonly contributes to left ventricular diastolic dysfunctionP237Intrinsic left ventricular dysfunction in Behcet disease in comparison with systemic disease activity: insights from speckle tracking echocardiographyP238Echocardiography changes during singleton and twin pregnancyP239Proposal of two new echocardiographic parameters for the differentiation of pre-capillary from post-capillary pulmonary hypertensionP240The coincidence of implanted device and number of electrodes with the presence of residual fibrotic tissue after transvenous leads extraction assessed in echocardiographic examP241Hemoglobin is an independent predictor of left ventricular hypertrophy in postmenopausal women but not in premenopausal womenP242Left ventricular longitudinal deformation impairment in patients with psoriasis is linked with immunologic activationP243Impaired diastolic functions and left atrial mechanical functions in patients with vitamin-D deficiencyP244Modification of cardiac and vascular function secondary to insulin resistanceP245Impaired right ventricular function is not related to serum galectin-3 concentration in patients with repaired tetralogy of fallotP246Age-adjusted indices of right ventricular (RV) longitudinal function do not adequately reflect the global RV contraction in children with repaired congenital heart defects and RV volume overloadP247Relationship between fractional flow reserve and dobutamine stress echocardiography in coronary artery diseaseP248A retrospective observational comparative study on the negative predictive value of nuclear testing vs. stress echo in pre-operative assessment for patients undergoing solid organ transplantionP249The detection of viable myocardium by dobutamine stress speckle tracking echocardiography in patients with coronary artery diseaseP250VO2 flattening during exercise in heart failure: evidence for a combined low cardiac output and inefficient O2 extractionP251 The dynamic assessment of alveolar-capillary barrier during exercise-echocardiography in heart failure patients with reduced ejection fractionP252The effect of exercise training on cardiac function during exercise stress echo in patients with type 2 diabetes and diastolic dysfunctionP253Age-related maximal exercise O2 extraction differences in a population of apparently healthy subjects at cardiovascular riskP254Difference in the changes of functional mitral regurgitation between semisupine ergometer and handgrip exerciseP255Correlation of annulus size assessed by echo 2d, 3d and multidetector computed tomography in patients undergoing transcatheter aortic valve implantationP256Mitral annulus dynamics: from normal to extensive mixomatous disease-a three-dimensional transoesophageal studyP257Left atrial appendage closure: an echo point of view of 55 casesP258Effect of catheter-based renal denervation on left ventricular function, mass and (un)twist with two-dimensional speckle tracking echocardiographyP259Associations of microRNAs gene expression in peripheral blood mononuclear cells and left ventricular global longitudinal peak strain in patients with essential hypertension [O] . I. Craciunescu, N. Ngiam, BJ. Sun, 2016

机译：心肌变形之间P235Relation通过三维斑点追踪分析，在患者全身hypertensionP236Obstructive睡眠呼吸暂停的高血压和功能的能力控制是经常根据公认在急性心肌梗死，但通常有助于左室舒张dysfunctionP237Intrinsic左心室功能紊乱中白塞氏病与全身性疾病的活性的比较：用于预毛细管的从植入装置和电极数目的毛细血管后肺hypertensionP240The巧合的分化与存在的两个新的超声心动图参数单和双pregnancyP239Proposal期间从斑点追踪echocardiographyP238Echocardiography变化的见解静脉引线后残留的纤维组织提取评估了超声心动图examP241Hemoglobin是绝经后妇女的左心室肥厚的独立预测因素，但不是在premen opausal womenP242Left心室纵向变形损害牛皮癣患者与免疫联activationP243Impaired舒张功能和左心房机械功能患者的心脏和血管功能继发于胰岛素的维生素-d deficiencyP244Modification resistanceP245Impaired右心室功能是不相关的血清半乳凝素3浓度患者右心室（RV）的纵向功能fallotP246Age调整指标的修复四部曲没有充分反映在冠状动脉diseaseP248A追溯血流储备分数和多巴酚丁胺超声心动图之间修复先天性心脏缺陷和RV容积overloadP247Relationship全球儿童RV收缩在术前评估核试验对应力回声的阴性预测值进行可行myocard的实体器官transplantionP249The检测患者的观察对比研究鎓通过多巴酚丁胺斑点跟踪超声心动图在患者的心脏衰竭锻炼期间冠状动脉diseaseP250VO2压扁：证据为一个组合的低心输出量和低效率的O2 extractionP251在心脏衰竭患者运动超声心动图中肺泡 - 毛细血管屏障的动态评估具有降低的喷射对心脏功能的运动锻炼的运动负荷超声过程中患者fractionP252The影响2型糖尿病患者和表面健康受试者的群体在心血管riskP254Difference在功能性二尖瓣返流的semisupine测力计和手柄之间的变化舒张dysfunctionP253Age相关的最大运动O2提取差异环大小的exerciseP255Correlation由回波2D评估，3d和多排的患者正在接受经导管主动脉瓣implantationP256Mitral环动力学计算机断层：从正常到广泛mixomatous疾病三dimensi Onal地区食道studyP257Left心耳闭合：视在左心室功能，质量和（UN）捻基于导管的肾去神经支配的55 casesP258Effect的在外周血单核细胞和左侧的微小RNA基因表达的二维散斑跟踪echocardiographyP259Associations回波点心室全球纵向应变峰值在原发性高血压患者

Mechanical dynamics of single cells during early apoptosis.

摘要

著录项

相似文献

相关主题

期刊订阅