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首页> 外文期刊>Cell motility and the cytoskeleton >Mechanical properties of the passive sea urchin sperm flagellum.
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Mechanical properties of the passive sea urchin sperm flagellum.

机译：被动型海胆精子鞭毛的力学性能。

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In this study we used Triton X-100 extracted sea urchin spermatozoa to investigate the mechanical behavior of the basic 9+2 axoneme. The dynein motors were disabled by vanadate so that the flagellum is rendered a passive structure. We find that when a proximal portion of the flagellum is bent with a glass microprobe, the remainder of the flagellum distal to the probe exhibits a bend in the opposite direction (a counterbend). The counterbend can be understood from the prevailing sliding doublet model of axoneme mechanics, but does require the existence of elastic linkages between the outer doublets. Analysis of the shapes of counterbends provides a consensus value of 0.03-0.08/microm(2) for the ratio of the interdoublet shear resistance (E(S)) to the bending resistance (E(B)) and we find that the ratio E(S)/E(B) is relatively conserved for both passive flagella and transiently quiescent live flagella. This ratio expresses a fundamental mechanical property of the eukaryotic axoneme. It defines the contributions to total bending resistance derived from bending the microtubules and from stretching the interdoublet linkages, respectively. Using this ratio, and computer simulations of earlier experiments that measured the total stiffness of the flagellum, we obtain estimates of approximately 1 x 10(8) pN nm(2)/rad for E(B) and 6 pN/rad for E(S), assuming that both elasticities are linear. Our results indicate that the behavior of the flagellum is close to that predicted by a linear model for shear elasticity.
机译：在这项研究中，我们使用Triton X-100提取的海胆精子来研究基本9 + 2轴突酶的力学行为。电动马达因钒酸盐而失效，从而使鞭毛成为被动结构。我们发现，当鞭毛的近端部分用玻璃微探针弯曲时，鞭毛在探针远侧的其余部分会在相反的方向上出现弯曲（反向弯曲）。可以从流行的轴突力学双联模型中了解到对接弯，但确实需要在外部双联之间存在弹性连接。配合弯曲形状的分析提供了一个双重抗剪强度（E（S））与抗弯强度（E（B））之比的0.03-0.08 / microm（2）共识值，我们发现比值E （S）/ E（B）对于被动鞭毛和暂时静止的活鞭毛均相对保守。该比例表示真核轴突的基本机械性能。它定义了对总抗弯强度的贡献，这些总抗弯强度分别来自于微管的弯曲和双链对联键的拉伸。使用该比率，以及较早进行的测量鞭毛总硬度的实验的计算机模拟，我们得出E（B）约为1 x 10（8）pN nm（2）/ rad，E（B）约为6 pN / rad。 S），假设两个弹性都是线性的。我们的结果表明，鞭毛的行为与剪切弹性线性模型预测的行为接近。

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