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首页> 外文期刊>Cell motility and the cytoskeleton >The roles of actin cytoskeleton and microtubules for membrane recycling of a food vacuole in Tetrahymena thermophila.
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The roles of actin cytoskeleton and microtubules for membrane recycling of a food vacuole in Tetrahymena thermophila.

机译：肌动蛋白细胞骨架和微管在嗜热四膜膜食物膜的膜回收中的作用。

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Phagocytosis is a fundamental cellular event for the uptake of nutrients from the environment in several kinds of eukaryote. Most ciliates egest waste and undigested materials in food vacuoles (FVs) through a cytoproct, which is a specific organelle for defecation. It is considered that FV egestion is initiated by fusion between the FV membrane and plasma membrane in a cytoproct and completed with retrieval of the membrane into a cytoplasmic space. In addition, electron microscopy indicated that microfilaments might be involved in the recycling process of the FV membrane in ciliates over 30 years ago; however, there is no conclusive evidence. Here we demonstrated actin organization on FV near a cytoproct in Tetrahymena thermophila by using a marker for a cytoproct. Moreover, it was revealed that cells treated with actin cytoskeletal inhibitor, Latrunculin B, might be suppressed for membrane retrieval in a cytoproct following FV egestion. On the other hand, the actin structures, likely to be the site of membrane retrieval, were frequently observed in the cells treated with cytoplasmic microtubules inhibitor, Nocodazole. We concluded that actin filaments were probably required for recycling of the FV membrane in a cytoproct although the role was not essential for FV egestion. In addition, it was possible that microtubules might be involved in transportation of recycling vesicles of FV coated with F-actin.
机译：吞噬作用是从几种真核生物中吸收环境中营养物质的基本细胞事件。多数纤毛通过食物过程中的排泄物，即食物液泡（FV）中的浪费和未消化的物质而产生。认为FV分离是通过在细胞过程中FV膜和质膜之间的融合而开始的，并通过将膜收回到细胞质空间中而完成。此外，电子显微镜显示，三十多年前，微丝可能参与了纤​​毛中FV膜的回收过程。但是，没有确凿的证据。在这里，我们通过使用嗜热性膜膜炎的标记，证明了肌动蛋白在嗜热四膜菌中靠近细胞过程的肌动蛋白组织。此外，揭示了用肌动蛋白细胞骨架抑制剂Latrunculin B处理的细胞在FV分离后可抑制细胞过程中的膜恢复。另一方面，在细胞质微管抑制剂诺考达唑处理的细胞中经常观察到肌动蛋白结构，很可能是膜修复的部位。我们的结论是，肌动蛋白丝可能是细胞过程中FV膜再循环的必需物质，尽管该作用对于FV分离并不重要。此外，微管可能参与了涂有F-肌动蛋白的FV回收囊泡的运输。

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