• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Vascular smooth muscle cell phenotypic modulation in culture is associated with reorganisation of contractile and cytoskeletal proteins.
【24h】

Vascular smooth muscle cell phenotypic modulation in culture is associated with reorganisation of contractile and cytoskeletal proteins.

机译：培养中的血管平滑肌细胞表型调节与收缩蛋白和细胞骨架蛋白的重组有关。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Smooth muscle cells (SMC) exhibit a functional plasticity, modulating from the mature phenotype in which the primary function is contraction, to a less differentiated state with increased capacities for motility, protein synthesis, and proliferation. The present study determined, using Western analysis, double-label immunofluorescence and confocal microscopy, whether changes in phenotypic expression of rabbit aortic SMC in culture could be correlated with alterations in expression and distribution of structural proteins. "Contractile" state SMC (days 1 and 3 of primary culture) showed distinct sorting of proteins into subcellular domains, consistent with the theory that the SMC structural machinery is compartmentalised within the cell. Proteins specialised for contraction (alpha-SM actin, SM-MHC, and calponin) were highly expressed in these cells and concentrated in the upper central region of the cell. Vimentin was confined to the body of the cell, providing support for the contractile apparatus but not co-localising with it. In line with its role in cell attachment and motility, beta-NM actin was localised to the cell periphery and basal cortex. The dense body protein alpha-actinin was concentrated at the cell periphery, possibly stabilising both contractile and motile apparatus. Vinculin-containing focal adhesions were well developed, indicating the cells' strong adhesion to substrate. In "synthetic" state SMC (passages 2-3 of culture), there was decreased expression of contractile and adhesion (vinculin) proteins with a concomitant increase in cytoskeletal proteins (beta-non-muscle [NM] actin and vimentin). These quantitative changes in structural proteins were associated with dramatic changes in their distribution. The distinct compartmentalisation of structural proteins observed in "contractile" state SMC was no longer obvious, with proteins more evenly distributed throughout the cytoplasm to accommodate altered cell function. Thus, SMC phenotypic modulation involves not only quantitative changes in contractile and cytoskeletal proteins, but also reorganisation of these proteins. Since the cytoskeleton acts as a spatial regulator of intracellular signalling, reorganisation of the cytoskeleton may lead to realignment of signalling molecules, which, in turn, may mediate the changes in function associated with SMC phenotypic modulation. Copyright 2001 Wiley-Liss, Inc.
机译：平滑肌细胞（SMC）具有功能可塑性，从主要功能是收缩的成熟表型调节到分化程度较低的状态，具有增强的运动性，蛋白质合成和增殖能力。本研究使用Western分析，双标记免疫荧光和共聚焦显微镜确定了培养物中兔主动脉SMC表型表达的变化是否与结构蛋白表达和分布的变化有关。 “收缩性”状态SMC（原代培养的第1天和第3天）显示出不同的蛋白质分类进入亚细胞结构域，这与SMC结构机制在细胞内分隔的理论一致。专门用于收缩的蛋白质（α-SM肌动蛋白，SM-MHC和钙还原蛋白）在这些细胞中高度表达，并集中在细胞的上部中央区域。波形蛋白被限制在细胞体内，为收缩装置提供支持，但并未与其共定位。根据其在细胞附着和运动中的作用，β-NM肌动蛋白位于细胞外围和基底皮质。致密的人体蛋白质α-肌动蛋白集中在细胞周围，可能稳定收缩和运动的设备。含Vinculin的粘着斑发育良好，表明细胞对基质的强粘着力。在“合成”状态SMC（培养2-3代）中，收缩和粘附（长蛋白）蛋白的表达降低，而细胞骨架蛋白（β非肌肉[NM]肌动蛋白和波形蛋白）随之增加。结构蛋白的这些定量变化与其分布的急剧变化有关。在“收缩”状态SMC中观察到的结构蛋白的明显区室化不再明显，蛋白质更均匀地分布在整个细胞质中以适应改变的细胞功能。因此，SMC表型调节不仅涉及收缩蛋白和细胞骨架蛋白的定量变化，而且还涉及这些蛋白的重组。由于细胞骨架充当细胞内信号传导的空间调节剂，因此细胞骨架的重组可能会导致信号分子重新排列，进而可能介导与SMC表型调节相关的功能变化。版权所有2001 Wiley-Liss，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号