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首页> 外文期刊>Cell motility and the cytoskeleton >Microtubule integrity is essential for apical polarization and epithelial morphogenesis in the thyroid.
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Microtubule integrity is essential for apical polarization and epithelial morphogenesis in the thyroid.

机译：微管完整性对于甲状腺的根尖极化和上皮形态发生至关重要。

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In this study, we examined the contribution of microtubules to epithelial morphogenesis in primary thyroid cell cultures. Thyroid follicles consist of a single layer of polarized epithelial cells surrounding a closed compartment, the follicular lumen. Freshly isolated porcine thyroid cells aggregate and reorganize to form follicles when grown in primary cultures. Follicular reorganization is principally a morphogenetic process that entails the assembly of biochemically distinct apical and basolateral membrane domains, delimited by tight junctions. The establishment of cell surface polarity during folliculogenesis coincided with the polarized redistribution of microtubules, predominantly in the developing apical poles of cells. Disruption of microtubule integrity using either colchicine or nocodazole caused loss of defined apical membrane domains, tight junctions and follicular lumina. Apical membrane and tight junction markers became randomly distributed at the outer surfaces of aggregates. In contrast, the basolateral surface markers, E-cadherin and Na(+),K(+)-ATPase, remained correctly localized at sites of cell-cell contact and at the free surfaces of cell aggregates. These findings demonstrate that microtubules play a necessary role in thyroid epithelial morphogenesis. Specifically, microtubules are essential to preserve the correct localization of apical membrane components within enclosed cellular aggregates, a situation that is also likely to pertain where lumina must be formed from solid aggregates of epithelial precursors. Copyright 2001 Wiley-Liss, Inc.
机译：在这项研究中，我们检查了微管对甲状腺原代细胞培养中上皮形态发生的贡献。甲状腺滤泡由单层极化的上皮细胞围绕着一个封闭的腔室即滤泡内腔组成。在原代培养中生长时，新鲜分离的猪甲状腺细胞聚集并重组形成卵泡。滤泡重组主要是一个形态发生过程，需要生化上独特的顶膜和基底外侧膜结构域的组装，并由紧密的连接点界定。卵泡形成过程中细胞表面极性的建立与微管的极化再分布相吻合，主要发生在细胞的发育中。使用秋水仙碱或诺考达唑破坏微管完整性会导致确定的根尖膜结构域，紧密连接和滤泡腔丢失。顶端的膜和紧密的连接标记变得随机分布在聚集体的外表面。相反，基底外侧表面标志物E-钙粘着蛋白和Na（+），K（+）-ATPase仍然正确地定位在细胞与细胞接触的位置以及细胞聚集体的自由表面上。这些发现表明，微管在甲状腺上皮形态发生中起必要的作用。特别地，微管对于保持封闭的细胞聚集体中的根尖膜成分的正确定位至关重要，这种情况也可能与必须由上皮前体的固体聚集体形成腔的情况有关。版权所有2001 Wiley-Liss，Inc.

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