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首页> 外文期刊>Lab on a chip >A fluidic device to study directional angiogenesis in complex tissue and organ culture models
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A fluidic device to study directional angiogenesis in complex tissue and organ culture models

机译：研究复杂组织和器官培养模型中定向血管生成的流体装置

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摘要

Many signals that induce angiogenesis have been identified; however, it is still not clear how these signals interact to shape the vascular system. We have developed a fluidic device for generation of molecular gradients in 3-dimensional cultures of complex tissues and organs in order to create an assay for precise induction and guidance of growing blood vessels. The device features a centrally placed culture chamber, flanked by channels attached to a perfusion system used to generate gradients. A separate network of vacuum channels permits reversible attachment of the device to a flat surface. We show that the fluidic device can be used to create growth factor gradients that induce directional angiogenesis in embryonic mouse kidneys and in clusters of differentiating stem cells. These results demonstrate that the device can be used to accurately manipulate complex morphogenetic processes with a high degree of experimental control.
机译：已经发现了许多诱导血管生成的信号。然而，目前尚不清楚这些信号如何相互作用以塑造血管系统。我们已经开发出一种用于在复杂组织和器官的3维培养物中生成分子梯度的流体装置，以便创建一种用于精确诱导和引导正在生长的血管的测定法。该设备的特点是位于中央的培养室，两侧是与用于产生梯度的灌注系统相连的通道。单独的真空通道网络可将设备可逆地连接到平坦表面。我们表明，该流体装置可用于创建生长因子梯度，诱导胚胎小鼠肾脏和分化干细胞簇中的定向血管生成。这些结果表明，该装置可用于以高度的实验控制来精确地操纵复杂的形态发生过程。

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