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首页> 外文期刊>Cell motility and the cytoskeleton >Reorganization and translocation of the ectoplasmic cytoskeleton in the leech zygote by condensation of cytasters and interactions of dynamic microtubules and actin filaments.
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Reorganization and translocation of the ectoplasmic cytoskeleton in the leech zygote by condensation of cytasters and interactions of dynamic microtubules and actin filaments.

机译：水condensation的凝结以及动态微管和肌动蛋白丝的相互作用使水the合子中的胞质细胞骨架重组和易位。

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摘要

The formation and bipolar translocation of an ectoplasmic cytoskeleton of rings and meridional bands was studied in interphase zygotes of the glossiphoniid leech Theromyzon trizonare. Zygotes consisted of a peripheral organelle-rich ectoplasm and an internal yolk-rich endoplasm. After microinjection of labeled tubulin and/or actin, zygotes were examined by time-lapse video imaging, immunofluorescence and confocal microscopy. The rings and meridional bands were formed by condensation of a network of moving cytasters that represented ectoplasmic secondary centers of microtubule and actin filament nucleation. In some cases the network of cytasters persisted between the rings. The cytoskeleton had an outer actin layer and an inner microtubule layer that merged at the irregularly-shaped boundary zone. Bipolar translocation of the rings, meridional bands, or the network of cytasters led to accumulation of the cytoskeleton at both zygote poles. Translocation of the cytoskeleton was slowed or arrested by microinjected taxol or phalloidin, in a dose-dependent fashion. Results of drug treatment probably indicate differences in the degree and speed at which the cytoskeleton becomes stabilized. Moreover, drugs that selectively stabilized either microtubules or actin filaments stabilized and impaired movement of the entire cytoskeleton. Microtubule poisons and latrunculin-B failed to disrupt the cytoskeleton. It is concluded that the microtubule and actin cytoskeletons are dynamic, presumably cross-linked and resistant to depolymerizing drugs. They probably move along each other by a sliding mechanism that depends on the instability of microtubules and actin filaments. Cell Motil. Cytoskeleton 53:214-230, 2002.
机译：研究了舌兰水echTheromyzon trizonare的相间合子中环和子午带的胞质细胞骨架的形成和双极易位。合子由周围富含细胞器的外质和内部富含卵黄的内质组成。显微注射标记微管蛋白和/或肌动蛋白后，通过延时视频成像，免疫荧光和共聚焦显微镜检查合子。环和子午带是由运动的cytaster网络的缩合形成的，cytasters网络代表微管的胞质次级中心和肌动蛋白丝的成核。在某些情况下，cytaster网络在环之间持续存在。细胞骨架具有在不规则形状的边界区域融合的外部肌动蛋白层和内部微管层。环，子午带或cytaster网络的双极易位导致两个合子极的细胞骨架积聚。通过微注射紫杉醇或鬼笔环肽以剂量依赖的方式减缓或阻止了细胞骨架的移位。药物治疗的结果可能表明细胞骨架稳定的程度和速度不同。而且，选择性地稳定微管或肌动蛋白丝的药物稳定并损害了整个细胞骨架的运动。微管毒物和latrunculin-B未能破坏细胞骨架。结论是微管和肌动蛋白的细胞骨架是动态的，大概是交联的并且对解聚药物具有抗性。它们可能通过取决于微管和肌动蛋白丝不稳定性的滑动机制彼此移动。细胞动力。 Cytoskeleton 53：214-230，2002。

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