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首页> 外文期刊>Cell motility and the cytoskeleton >Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation.
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Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation.

机译：酵母多肽链释放因子eRF1和eRF3参与细胞骨架组织和细胞周期调控。

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摘要

Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRF1 and eRF3. eRF1 recognizes nonsense codons UAA, UAG, and UGA, while eRF3 stimulates polypeptide release from the ribosome in a GTP- and eRF1-dependent manner. In the yeast Saccharomyces cerevisiae, eRF1 and eRF3 are encoded by the SUP45 and SUP35 genes, respectively. Here we show that in yeast shortage of any one of the release factors was accompanied by a reduction in the levels of the other release factor and resulted in a substantial increase of nonsense codon readthrough. Besides, repression of the genes encoding these factors caused different effects on cell morphology. Repression of the SUP35 gene caused accumulation of cells of increased size with large buds. This was accompanied by the disappearance of actin cytoskeletal structures, impairment of the mitotic spindle structure, and defects in nuclei division and segregation in mitosis. The evolutionary conserved C-terminal domain of eRF3 similar to the elongation factor EF-1alpha was responsible for these effects. Repression of the SUP45 gene caused accumulation of unbudded cells with 2C and higher DNA content, indicating that DNA replication is uncoupled from budding. The data obtained suggest that eRF1 and eRF3 play additional, nontranslational roles in the yeast cell. Cell Motil. Cytoskeleton 52:161-173, 2002.
机译：真核生物中翻译的终止受两个相互作用的多肽链释放因子eRF1和eRF3控制。 eRF1识别无意义的密码子UAA，UAG和UGA，而eRF3则以GTP和eRF1依赖性方式刺激多肽从核糖体释放。在酵母酿酒酵母中，eRF1和eRF3分别由SUP45和SUP35基因编码。在这里，我们表明，在酵母菌中，任何一种释放因子的短缺都伴随着另一种释放因子的水平降低，并导致无义密码子通读率显着提高。此外，编码这些因子的基因的抑制对细胞形态产生不同的影响。 SUP35基因的压制导致具有大芽的尺寸增加的细胞积累。这伴随着肌动蛋白细胞骨架结构的消失，有丝分裂纺锤体结构的损害以及有丝分裂中核分裂和分离的缺陷。 eRF3的进化保守C末端域类似于伸长因子EF-1alpha负责这些作用。 SUP45基因的抑制导致2C和更高的DNA含量的未预算细胞的积累，表明DNA复制是与芽分离的。获得的数据表明，eRF1和eRF3在酵母细胞中还发挥了其他非翻译作用。细胞动力。细胞骨架52：161-173，2002。

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