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首页> 外文期刊>Lab on a chip >Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
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Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications

机译：微流控装置可研究影响为经皮应用设计的生物传感器性能的因素

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In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling chemistry. An epoxy-polyurethane based membrane was used to extend the linear working range (from 0 to 25mM of substrate) of these biosensors. Both lactate and glucose sensors performed well in the clinically relevant substrate concentration range. Glucose microspikes were further investigated with respect to the effects of substrate transfer by incorporation into a microfluidic system. Data from the microfluidic system revealed that the sensor response is mainly dependent on enzyme kinetics rather than membrane permeability to glucose. The robustness of the sensors was demonstrated by its consistency in performance extending over 48 h.
机译：在这项工作中，我们演示了一个新颖的基于微流体的平台，以研究基于3D平面外微钉阵列的葡萄糖和乳酸生物传感器的性能。将微钉阵列与载玻片结合，并使用共价偶联化学方法用葡萄糖氧化酶或乳酸氧化酶修饰。使用基于环氧-聚氨酯的膜来扩展这些生物传感器的线性工作范围（从0到25mM的底物）。乳酸和葡萄糖传感器在临床相关底物浓度范围内均表现良好。通过结合到微流体系统中，进一步研究了葡萄糖微峰关于底物转移的影响。来自微流体系统的数据显示，传感器响应主要取决于酶动力学，而不是膜对葡萄糖的渗透性。传感器的耐用性通过其在48小时内的性能一致性来证明。

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