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首页> 外文期刊>Lab on a chip >Fabrication of a cyclic olefin copolymer planar waveguide embedded in a multi-channel poly(methyl methacrylate) fluidic chip for evanescence excitation
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Fabrication of a cyclic olefin copolymer planar waveguide embedded in a multi-channel poly(methyl methacrylate) fluidic chip for evanescence excitation

机译：嵌入多通道聚甲基丙烯酸甲酯流体芯片中用于消逝激发的环状烯烃共聚物平面波导的制备

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摘要

The fabrication and characterization of a novel cyclic olefin copolymer (COC) waveguide embedded in a poly(methyl methacrylate), PMMA, fluidic chip configured in a multi-channel format with an integrated monolithic prism for evanescent fluorescence excitation are reported. The fabrication approach allowed the embedded waveguide to be situated orthogonal to a series of fluidic channels within the PMMA wafer to sample fluorescent solutions in these channels using the evanescence properties of the waveguide. Construction of the device was achieved using several fabrication techniques including high precision micromilling, hot embossing and stenciling of a polymer melt to form the waveguide and coupling prism. A waveguide channel was fabricated in the fluidic chip's cover plate, also made from PMMA, and was loaded with a COC solution using a pre-cast poly(dimethylsiloxane), PDMS, stencil containing a prism-shaped recess. The PMMA substrate contained multiple channels (100 μm wide × 30 μm deep with a pitch of 100 μm) that were situated orthogonal to the waveguide to allow penetration of the evanescent field into the sampling solution. The optical properties of the waveguide in terms of its transmission properties and penetration depth of the evanescent field in the adjacent solution were evaluated. Finally, the device was used for laser-induced fluorescence evanescent excitation of a dye solution hydrodynamically flowing through multiple microfluidic channels in the chip and processed using a microscope equipped with a charge-coupled device (CCD) for parallel readout. The device and optical system were able to image 11 channels simultaneously with a limit-of-detection of 7.1 × 10~(-20) mol at a signal-to-noise ratio of 2. The waveguide was simple to manufacture and could be scaled to illuminate much higher channel numbers making it appropriate for high-throughput measurements using evanescent excitation.
机译：报道了一种新型的环状烯烃共聚物（COC）波导的制造和特性，该波导嵌入聚（甲基丙烯酸甲酯），PMMA，流体芯片中，该芯片以多通道形式配置，带有集成的整体棱镜，用于e逝荧光激发。该制造方法允许将嵌入式波导放置在与PMMA晶片内的一系列流体通道正交的位置，以使用波导的渐逝特性对这些通道中的荧光溶液进行采样。使用几种制造技术可实现设备的构造，包括高精度微铣削，热压花和对聚合物熔体进行模切以形成波导和耦合棱镜。在同样由PMMA制成的流体芯片的盖板中制造了一个波导通道，并使用预制的聚二甲基硅氧烷PDMS，包含棱柱形凹口的模版向其中加载了COC溶液。 PMMA基板包含多个通道（宽100μm×深30μm，间距为100μm），它们垂直于波导放置，以允许van逝场穿透到采样溶液中。根据波导的传输特性和the逝场在相邻溶液中的穿透深度，对波导的光学特性进行了评估。最后，该设备用于激光诱导荧光消逝激发染料溶液，该染料溶液在流体中动态流过芯片中的多个微流体通道，并使用配备有电荷耦合器件（CCD）的显微镜进行平行读数处理。该器件和光学系统能够在7.1×10〜（-20）mol的信噪比为2的情况下同时成像11个通道。波导易于制造并且可以按比例缩放照明更高的通道数，使其适合使用using逝激发的高通量测量。

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