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首页> 外文期刊>Lab on a chip >Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining
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Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining

机译：通过超精密微加工制造的流体动力学聚焦血细胞的流式细胞术分析的微流结构

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We present three-dimensional microfluidic structures with integrated optical fibers, mirrors and electrodes for flow cytometric analysis of blood cells. Ultraprecision milling technique was used to fabricate different flow cells featuring single-stage and two-stage cascaded hydrodynamic focusing of particles by a sheath flow. Two dimensional focussing of the sample fluid was proven by fluorescence imaging in horizontal and vertical directions and found to agree satisfactorily with finite element calculations. Focussing of the sample stream down to 5 urn at a particle velocity of 3 m s~(-1) is accessible while maintaining stable operation for sample flow rates of up to 20 mu L mm~(-1). In addition to fluorescence imaging, the micro-flow cells were characterised by measurements of pulse shapes and pulse height distributions of monodisperse microspheres. We demonstrated practical use of the microstructures for cell differentiation employing light scatter to distinguish platelets and red blood cells. Furthermore, T-helper lymphocytes labelled by monoclonal antibodies were identified by measuring side scatter and fluorescence.
机译：我们提出了具有集成的光纤，镜子和电极的三维微流体结构，用于血细胞的流式细胞术分析。使用超精密研磨技术来制造不同的流通池，这些流通池的特征是通过鞘流对颗粒进行单级和两级级联的流体动力学聚焦。通过在水平和垂直方向上的荧光成像证明了样品流体的二维聚焦，并且发现其与有限元计算令人满意。在3 m s〜（-1）的粒子速度下，可以将样品流聚焦至5 um，同时在高达20μL mm〜（-1）的样品流量下保持稳定的运行。除荧光成像外，微流动池还通过测量单分散微球的脉冲形状和脉冲高度分布来表征。我们证明了微结构在细胞分化中的实际应用，该过程通过光散射来区分血小板和红细胞。此外，通过测量侧向散射和荧光来鉴定被单克隆抗体标记的T辅助淋巴细胞。

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