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首页> 外文期刊>Cell motility and the cytoskeleton >Separating centrosomes interact in the absence of associated chromosomes during mitosis in cultured vertebrate cells.
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Separating centrosomes interact in the absence of associated chromosomes during mitosis in cultured vertebrate cells.

机译：在培养的脊椎动物细胞的有丝分裂过程中，分离的中心体在不存在相关染色体的情况下相互作用。

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We detail here how "free" centrosomes, lacking associated chromosomes, behave during mitosis in PtK(2) homokaryons stably expressing GFP-alpha-tubulin. As free centrosomes separate during prometaphase, their associated astral microtubules (Mts) interact to form a spindle-shaped array that is enriched for cytoplasmic dynein and Eg5. Over the next 30 min, these arrays become progressively depleted of Mts until the two centrosomes are linked by a single bundle, containing 10-20 Mts, that persists for > 60 min. The overlapping astral Mts within this bundle are loosely organized, and their plus ends terminate near its midzone, which is enriched for an ill-defined matrix material. At this time, the distance between the centrosomes is not defined by external forces because these organelles remain stationary when the bundle connecting them is severed by laser microsurgery. However, since the centrosomes move towards one another in response to monastrol treatment, the kinesin-like motor protein Eg5 is involved. From these results, we conclude that separating asters interact during prometaphase of mitosis to form a spindle-shaped Mt array, but that in the absence of chromosomes this array is unstable. An analysis of the existing data suggests that the stabilization of spindle Mts during mitosis in vertebrates does not involve the chromatin (i.e., the RCC1/RanGTP pathway), but instead some other chromosomal component, e.g., kinetochores. Cell Motil. Cytoskeleton 52:107-121, 2002.
机译：我们在这里详细描述了缺少关联染色体的“自由”中心体在稳定表达GFP-α-微管蛋白的PtK（2）同核体的有丝分裂过程中的行为。由于游离中心体在前中期分离，它们相关的星状微管（Mts）相互作用形成纺锤形阵列，富含细胞质动力蛋白和Eg5。在接下来的30分钟内，这些阵列逐渐耗尽Mts，直到两个中心体通过包含10-20 Mts的单个束连接起来，并持续> 60分钟。该束中重叠的星空Mt松散地组织起来，它们的正端在其中间区域附近终止，该区域充斥着不确定的基质材料。此时，中心体之间的距离不受外力的限制，因为当通过激光显微外科手术切断连接它们的束时，这些细胞器保持静止。但是，由于中心体响应monastrol治疗而相互靠近，因此涉及到了类似驱动蛋白的运动蛋白Eg5。从这些结果，我们得出结论，分离的翠菊在有丝分裂的前期相互作用，形成纺锤形的Mt阵列，但是在没有染色体的情况下，该阵列是不稳定的。对现有数据的分析表明，脊椎动物有丝分裂期间纺锤体Mts的稳定不涉及染色质（即RCC1 / RanGTP途径），而是涉及其他一些染色体成分，例如动植物。细胞动力。 Cytoskeleton 52：107-121，2002。

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