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首页> 外文期刊>Cell motility and the cytoskeleton >Quantitative analysis of the behavior of Dictyostelium discoideum amoebae: stringency of pteridine reception.
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Quantitative analysis of the behavior of Dictyostelium discoideum amoebae: stringency of pteridine reception.

机译：变形双歧杆菌的行为的定量分析：严格接受蝶啶。

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摘要

A convenient, sensitive, quantitative assay for the measurement of chemotaxis of populations of D. discoideum vegetative amoebae is presented. A strategy for determining the boundary of the bulk of a population of migrating amoebae was devised and is described. This assay employs a dynamic gradient and is independent of deaminase activity. Measurements of chemoattractant capabilities of various pteridines, folates, and mixtures of folate fragments are reported. 2-Amino 4-quinazolinone, a pterin analog without the pyrazine ring nitrogens, is chemotactic. Lumazine, deaminated pterin, inhibits chemotaxis towards pterin but not towards folic acid. Deaminofolic acid is a chemoattractant as are mixtures of lumazine plus aminobenzoylglutamic acid or deaminopteroic acid plus various amino acids. Separately, the components of these mixtures exhibit no ability to stimulate chemotaxis. These mixtures are of fragments that together comprise most of the folate structure. Our results are in accord with separate receptors for pterin vs. folic acid and with a high stringency for pterin reception but a relative tolerance for folate reception. The possibility of using such mixtures to investigate the requirements of various parts of the folate structure for competent signalling is discussed. Copyright 2002 Wiley-Liss, Inc.
机译：提出了一种方便，灵敏的定量测定法，用于测定迪斯科念珠菌营养性变形虫的趋化性。设计并描述了一种确定迁移的变形虫种群的边界的策略。该测定采用动态梯度，并且与脱氨酶活性无关。报道了各种蝶啶，叶酸和叶酸片段混合物的化学吸引能力的测量。 2-氨基4-喹唑啉酮是没有吡嗪环氮的蝶呤类似物，具有趋化性。去甲蝶呤Lumazine抑制对蝶呤的趋化作用，但对叶酸没有抑制作用。脱氨叶酸是化学吸引剂，如鲁嗪与氨基苯甲酰谷氨酸或脱氨基蝶酸加各种氨基酸的混合物。分别地，这些混合物的组分不表现出刺激趋化性的能力。这些混合物是一起组成大部分叶酸结构的碎片。我们的结果与蝶呤和叶酸的单独受体一致，对蝶呤的接受具有严格的要求，但对叶酸的接受具有相对的耐受性。讨论了使用此类混合物研究叶酸结构各个部分对有效信号传导的要求的可能性。版权所有2002 Wiley-Liss，Inc.

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