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首页> 外文期刊>Cell motility and the cytoskeleton >Reconstruction of the projection periodicity and surface architecture of the flagellar central pair complex.
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Reconstruction of the projection periodicity and surface architecture of the flagellar central pair complex.

机译：重建鞭毛中央对复合体的投影周期和表面结构。

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The substructure of central pair microtubule-associated components has been analyzed by comparing thin section and freeze-etch images of Chlamydomonas flagellar axonemes. The longitudinal periodicity of central pair projections that were previously described from cross-sectional image averages was determined from thin sections of axonemes isolated from either wild type or central pair assembly-defective strains. All projections directed toward one quadrant of the central pair repeat at 32 nm, while those in the other three quadrants all show 16-nm spacing. The surface architecture of these projections as seen in rapid-freeze deep-etch images of central pair complexes includes elements that form circumferentially oriented fibers around most of the central pair. This appearance changes dramatically along the lateral edge of the C1 microtubule where material is arranged in rows of separate particles that may play a unique role in spoke-mediated regulation of flagellar dynein activity. Cell Motil. Cytoskeleton 55:188-199, 2003.
机译：中央对微管相关组件的亚结构已通过比较薄层衣藻和鞭毛轴突的冷冻蚀刻图像进行了分析。先前从横截面图像平均值中描述的中央对投影的纵向周期性是根据从野生型或中央对装配缺陷型菌株中分离的轴突的薄层确定的。指向中心对的一个象限的所有投影在32 nm处重复，而其他三个象限中的所有投影都显示16 nm的间距。从中心对复合体的快速冻结深蚀刻图像中可以看到，这些投影的表面结构包括围绕大部分中心对形成圆周定向纤维的元素。这种外观沿C1微管的侧向边缘发生了巨大变化，其中材料排列在成排的独立颗粒中，这些颗粒可能在辐条介导的鞭毛动力蛋白调控中发挥独特作用。细胞动力。 Cytoskeleton 55：188-199，2003。

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