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首页> 外文期刊>Laboratory Animal Science >DETECTION OF CLOSTRIDIUM DIFFICILE TOXINS FROM THE SMALL INTESTINE AND CECUM OF RABBITS WITH NATURALLY ACQUIRED ENTEROTOXEMIA
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DETECTION OF CLOSTRIDIUM DIFFICILE TOXINS FROM THE SMALL INTESTINE AND CECUM OF RABBITS WITH NATURALLY ACQUIRED ENTEROTOXEMIA

机译：从小肠和自然采肠毒素的兔子的C中检测出艰难梭菌毒素

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Four specific-pathogen-free rabbits with anorexia died peracutely; decreased fecal output, nasal exudate, and labored breathing were the only other clinical abnormalities observed in two of the rabbits before death. The animals, three juveniles and one adult, were on a standard polyclonal antibody production regimen and had received immunizations approximately 2 weeks before presentation. External examination revealed distended abdomen and perineal fecal staining, At necropsy the small intestine was distended with fluid, and the cecum was distended with chyme. The small intestines and cecum had marked serosal hyperemia. Anaerobic bacterial culture techniques were used to isolate Clostridium difficile from the small intestine (3/4) and cecum (2/4). In all cases C. difficile toxin B was detected at high titers (10(2) to >10(5)) in the small intestine by cytotoxicity assay with HeLa 229 cell culture, In two of the four rabbits C. difficile was isolated, and cytotoxin titers were detected at 10(1) and 10(4) in the cecum of affected rabbits. Toxin B was neutralized with C. sordellii antiserum but not C. spiroforme antiserum, In addition, toxin A was detected in each of the cytotoxin B-positive samples by a commercial toxin A enzyme immunosorbent assay. In vitro production of toxins A and B was detected from each culture isolate after incubation in chopped meat broth. These cases are noteworthy because spontaneous (nonantibiotic-associated) C. difficile enterotoxemia has not been previously reported in rabbits. Also the toxins of clostridial organisms are usually documented in the cecum, not the small intestine, of rabbits. On the basis of these findings, the differential diagnosis of peracute mortality in colony-maintained rabbits should include C. difficile- as well as C. spiroforme-induced enterotoxemia.
机译：四只无特定病原体的厌食症兔子彻底死亡。粪便输出减少，鼻液渗出和呼吸困难是在死前两只兔子中观察到的仅有的其他临床异常。这些动物，三只少年和一只成年动物，采用标准的多克隆抗体生产方案，并在接种前约2周接受了免疫。外部检查显示腹部和会阴粪便染色肿大，尸检时小肠充满液体，盲肠肿大食糜。小肠和盲肠有明显的浆膜充血。厌氧细菌培养技术用于从小肠（3/4）和盲肠（2/4）分离艰难梭菌。在所有情况下，通过HeLa 229细胞培养物的细胞毒性试验，在小肠中以高滴度（10（2）至> 10（5））检测到艰难梭菌毒素B，在四只兔子中，有两只分离出艰难梭菌，并在患兔盲肠的10（1）和10（4）检测到细胞毒素滴度。毒素B用C. sordellii抗血清中和，而螺旋藻抗血清不中和。此外，通过商业毒素A酶免疫吸附测定法在每个细胞毒素B阳性样品中检测到毒素A。在切碎的肉汤中孵育后，从每种培养物分离物中检测到毒素A和B的体外产生。这些病例值得注意，因为以前没有在兔中报告过自发（非抗生素相关性）艰难梭菌肠毒素血症。梭菌的毒素通常也记录在兔子的盲肠而不是小肠中。基于这些发现，对集落养兔的全死率的鉴别诊断应包括艰难梭菌和螺旋形梭菌引起的肠毒血症。

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