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首页> 外文期刊>Cell motility and the cytoskeleton >The molecular biology of Euglena gracilis. XV. Recovery from centrifugation-induced stratification.
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The molecular biology of Euglena gracilis. XV. Recovery from centrifugation-induced stratification.

机译：Euglena gracilis的分子生物学。 XV。从离心诱导的分层中恢复。

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摘要

The contents of Euglena gracilis cells can be separated in vivo by ultracentrifugation. Within the unbroken cell, each set of components forms a distinct layer according to their respective densities. The degree of segregation increases with both the g-force and the time of centrifugation, up to a maximum at 100,000 x g for 1 h, when six distinct strata can be observed. When returned to normal growth conditions, essentially all the cells return to the normal state and growth pattern. Greater g-forces or longer exposures do not alter the observable strata, but the ability of the cells to recover is diminished. Smaller g-forces result in less separation of cellular contents and all cells recover, even after 18 h of exposure. Euglena cells stratified at 100,000 x g for 1 h were returned to normal growth conditions; recovery was followed microscopically and by the rate of utilization of oxygen as well as that of the single carbon source. The cells recovered their normal state within 1 to 2 h, which is onlya tenth of the normal doubling time. The mechanism for this recovery involves a natural process of change in cell shape caused by contraction and relaxation of the pellicle, a cell surface structure. Cell Motil. Cytoskeleton 56:219-224, 2003. Published 2003 Wiley-Liss, Inc.
机译：可以通过超速离心在体内分离Euglena gracilis细胞的含量。在不间断的单元中，每组组件根据其各自的密度形成一个不同的层。当可以观察到六个不同的层时，偏析程度随g力和离心时间的增加而增加，在100,000 x g的条件下最大持续1 h。当恢复到正常生长条件时，基本上所有细胞都恢复到正常状态和生长方式。更大的重力或更长的暴露时间不会改变可观察到的层，但是细胞恢复的能力却降低了。较小的g力导致较少的细胞内含物分离，即使暴露18 h，所有细胞也会恢复。以100,000×g分层1小时的Euglena细胞恢复到正常的生长条件。显微镜下观察回收率，其次是氧气和单碳源的利用率。细胞在1至2小时内恢复了正常状态，仅是正常倍增时间的十分之一。这种恢复的机制涉及由表膜的收缩和松弛（细胞表面结构）引起的细胞形状变化的自然过程。细胞动力。 Cytoskeleton 56：219-224，2003年。2003年发布，Wiley-Liss，Inc.

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