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首页> 外文期刊>Cell motility and the cytoskeleton >Erythroid spectrin in miceller detergents.
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Erythroid spectrin in miceller detergents.

机译：胶束清洁剂中的类红蛋白血影素。

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摘要

We have studied the interaction of spectrin, the major protein of the erythrocyte cytoskeleton, with four commonly used detergents at concentrations above their critical miceller concentrations (cmc). Fluorescence spectroscopic studies on the emission intensity, steady state polarization, quenching with acrylamide, and time-resolved fluorescence measurements were done with spectrin in anionic detergents, e.g., SDS, deoxycholate, and nonionic detergents, e.g., Triton-X-100 and octylglucoside at concentrations double their respective cmc's. The spectrin-detergent complexes in all four systems have been characterized by far-UV CD and measurements on tryptophan fluorescence in combination with fluorescence of the extrinsic probe, pyrene. Tryptophan fluorescence studies revealed quaternary structural changes due to unzipping of the spectrin subunits in Triton-X-100 without complete dissociation. Both Triton-X-100 and SDS were found to partially denature spectrin indicated by the far-UV CD. Octylglucoside and deoxycholate are shown to have the least structural perturbations on the cytoskeletal protein, rationalizing the use of octylglucoside, in particular and also deoxycholate to be the most effective in preparing cytoskeletal fractions from erythrocytes rather than the Triton-X-100 that has long been used for preparing the Triton shells. Cell Motil. Cytoskeleton 54:16-28, 2003.
机译：我们研究了血红蛋白（红细胞骨架的主要蛋白质）与四种常用去污剂（其临界胶束浓度（cmc）以上）的相互作用。荧光光谱研究了在去污剂（如SDS，脱氧胆酸盐和非离子去污剂，如Triton-X-100和辛基葡糖苷）中用血影蛋白进行的发射强度，稳态极化，丙烯酰胺猝灭和时间分辨的荧光测量。浓度是其各自cmc的两倍。所有四个系统中的血影蛋白清洁剂复合物均已通过远紫外CD和色氨酸荧光测量以及外在探针pyr的荧光结合进行了表征。色氨酸荧光研究表明，由于Triton-X-100中的血影蛋白亚基解压缩，四级结构发生了变化，而没有完全解离。发现Triton-X-100和SDS都可以使远紫外CD指示的血影蛋白部分变性。辛基葡萄糖苷和脱氧胆酸盐显示出对细胞骨架蛋白的结构扰动最少，尤其是合理使用辛基葡萄糖苷，并且脱氧胆酸盐是最有效的从红血球制备细胞骨架级分的方法，而不是长期以来一直以来的Triton-X-100用于准备Tri核弹。细胞动力。 Cytoskeleton 54：16-28，2003。

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