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首页> 外文期刊>Laboratory Animal Science >USE OF MAGNETIC CELL SORTING TO ISOLATE BLOOD NEUTROPHILS FROM RATS
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USE OF MAGNETIC CELL SORTING TO ISOLATE BLOOD NEUTROPHILS FROM RATS

机译：使用磁性细胞分级分离大鼠血液中性粒细胞

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摘要

The difficulty in obtaining sufficient numbers of neutrophils from rat blood limits the usefulness of this species in studies involving neutrophil function, To increase the neutrophil yield from rats drinking alcohol on a long-term basis, which further decreases neutrophil yield, we developed a magnetic cell-sorting technique, The rats were exsanguinated and neutrophils were isolated, using either traditional density gradient centrifugation or magnetic cell sorting, In the latter method, the leukocytes were labeled with biotinylated anti-rat granulocyte antibodies, followed by addition of streptavidin-conjugated superparamagnetic microbeads, The labeled cell suspension was applied to a steel wool column suspended within a magnetic field, Unlabeled cells were washed through the column, Retained, labeled neutrophils were eluted after the column was removed from the magnetic field, Compared with density gradient centrifugation, magnetic cell sorting yielded two-to fivefold higher neutrophil numbers per rat with increased purity, Viability was comparable for neutrophils isolated by the two techniques, Magnetic cell sorting is a rapid, gentle method for isolation of rat blood neutrophils and enhances the potential usefulness of rats in neutrophil-related research.
机译：从大鼠血液中获取足够数量的中性粒细胞的困难限制了该物种在涉及中性粒细胞功能的研究中的用途。为了长期增加饮酒大鼠的中性粒细胞产量，这进一步降低了中性粒细胞的产量，我们开发了磁性细胞分选技术，使用传统的密度梯度离心或磁性细胞分选法将大鼠放血并分离中性粒细胞。在后一种方法中，将白细胞标记有生物素化的抗大鼠粒细胞抗体，然后添加链霉亲和素偶联的超顺磁性微珠，将标记的细胞悬液应用于悬浮在磁场中的钢丝棉柱，未标记的细胞通过柱洗涤，保留的标记的中性粒细胞从磁场中移出后洗脱，与密度梯度离心相比，磁性细胞分选产生了2至5倍的中性粒细胞增多每只大鼠的纯度都有增加，通过两种技术分离出的中性粒细胞的生存力可比。磁性细胞分选是一种快速，温和的分离大鼠血液中性粒细胞的方法，并增强了大鼠在中性粒细胞相关研究中的潜在用途。

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