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首页> 外文期刊>Laboratory Animal Science >NEW IMMUNODEFICIENT MOUSE STRAINS BRED BY INTRODUCING BEIGE AND XID MUTATIONS INTO THE KSN NUDE STRAIN
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NEW IMMUNODEFICIENT MOUSE STRAINS BRED BY INTRODUCING BEIGE AND XID MUTATIONS INTO THE KSN NUDE STRAIN

机译：通过将米色和XID突变引入KSN裸体菌株而产生的新的免疫缺陷小鼠菌株

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摘要

We introduced two mutant genes (beige or bg, which induces a deficiency of natural killer activity, and xid, which decreases the production of immunoglobulins) into KSN nude mice with high reproductive performance, At first we produced the KSN-bg/bg(nuu) (KSN-bg) and KSN-xid/xid(nuu) (KSN-xid) congenic strain by backcross (cross-intercross method). After we identified homozygosity at the biochemical locus and polymorphic microsatellite loci, we mated KSN-bg and KSN-xid mice, and selected the KSN=xid/xid;bg/bg(nuu) (KSN-BNX) mice from the progeny, Furthermore we introduced the non-nude gene, which originated from CBA/N, into the KSN strain and produced KSN-nu/+ (KS) mice that have the same genetic background except for the nu locus. All strains had as high a reproductivity rate as the parental KSN mice. The KSN-xid and KSN-BNX mice had a reduced percentage of B220-positive cells in the spleen compared with KSN and KSN-bg mice, but they had increased percentages of Thy-1 and asialo GM1-positive cells. The serum immunoglobulin concentrations of BNX were as low as those of KSN-xid mice, Both KSN-bg and KSN-BNX mice had deficient natural killer activity in the spleen, whereas KSN-xid mice had increased natural killer activity. Compared with nude mice, the growth of the human thyroid tumor cell line transplanted subcutaneously was enhanced in BNX mice. These KSN, KSN-bg, KSN-xid, KSN-BNX, and KS mice not only are of value for use in various fields as the hosts of xenograft but also are good models of the combination effect of multiple immunodeficient genes.
机译：我们向具有高繁殖力的KSN裸鼠中引入了两个突变基因（米黄色或bg导致自然杀伤活性不足，而xid降低了免疫球蛋白的产生），首先我们产生了KSN-bg / bg（nu通过回交（交叉-交叉法）获得（KSN-bg）和KSN-xid / xid（nu / nu）（KSN-xid）同基因菌株。在我们确定了生化基因座和多态性微卫星基因座的纯合性后，我们将KSN-bg和KSN-xid小鼠交配，并从后代中选择了KSN = xid / xid; bg / bg（nu / nu）（KSN-BNX）小鼠，此外，我们将源自CBA / N的非裸基因导入KSN株，并生产了除了nu基因座外具有相同遗传背景的KSN-nu / +（KS）小鼠。所有品系均具有与亲本KSN小鼠一样高的繁殖率。与KSN和KSN-bg小鼠相比，KSN-xid和KSN-BNX小鼠的脾脏中B220阳性细胞百分比降低，但Thy-1和无唾液腺GM1阳性细胞百分比升高。 BNX的血清免疫球蛋白浓度与KSN-xid小鼠一样低，KSN-bg和KSN-BNX小鼠的脾脏自然杀伤力均不足，而KSN-xid小鼠的自然杀伤力却较高。与裸鼠相比，BNX小鼠皮下移植的人甲状腺肿瘤细胞系的生长得到了增强。这些KSN，KSN-bg，KSN-xid，KSN-BNX和KS小鼠不仅作为异种移植的宿主在各个领域具有重要价值，而且还是多种免疫缺陷基因联合效应的良好模型。

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