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首页> 外文期刊>Laboratory Animal Science >MUCUS SECRETAGOGUE ACTIVITY IN CECAL CONTENTS OF RABBITS WITH MUCOID ENTEROPATHY
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MUCUS SECRETAGOGUE ACTIVITY IN CECAL CONTENTS OF RABBITS WITH MUCOID ENTEROPATHY

机译：粘液性肠道病兔粪便中粘液分泌活性

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摘要

Cecal filtrates from rabbits with cecal ligation-induced mucoid enteropathy have been reported to cause goblet cell hyperplasia in intestinal explants. This study was performed to see whether such filtrates would stimulate mucus secretion from intestinal explants. Filtrates were prepared from cecal contents of five control rabbits, five rabbits that had undergone cecal ligation, and five rabbits that had had the distal branch of the ileocecocolic vessels and nerve incorporated into the cecal ligature. After incubation of each with ileal and colonic explants from five healthy rabbits, the amount of mucus in the media/filtrate solution was measured with an enzyme-linked lectin assay, Significantly (P < 0.05) more mucus was secreted by colonic explants in the presence of cecal filtrates from either of the cecal. ligation groups compared with controls, With pooled filtrates from each group, these results were confirmed by measuring secretion of radiolabeled mucus from explants that had been preincubated with [H-3]glucosamine. The secretagogue activity was found to be precipitable by 85% ammonium sulfate and destroyed by heat treatment (100 degrees C for 30 min) or acid treatment (pH 1.0 for 30 min), Very little, if any, secretagogue activity was present in cecal vein serum, The results support the hypothesis that the cecal contents of rabbits with cecal ligation-induced mucoid enteropathy stimulate mucus secretion from colonic explants taken from healthy rabbits.
机译：据报道，盲肠结扎诱导的粘液性肠病的兔子的盲肠滤液会引起肠道外植体杯状细胞增生。进行这项研究以观察这种滤液是否会刺激肠道外植体的粘液分泌。从五只对照兔子，五只进行盲肠结扎的兔子和五只兔子的盲肠内容物中制备滤液，其中五只兔子的回肠球菌血管远端和神经结合到盲肠结扎物中。将每只小鼠与来自五只健康兔子的回肠和结肠外植体孵育后，用酶联凝集素测定法测量培养基/滤液溶液中的粘液含量。在存在的情况下，结肠外植体分泌的粘液明显更多（P <0.05）来自任一盲肠的盲肠滤液。连接组与对照组相比，每组合并滤液，通过测量已用[H-3]葡糖胺预孵育的外植体中放射性标记的粘液的分泌，可以证实这些结果。发现促分泌素活性可被85％的硫酸铵沉淀并通过热处理（100℃30分钟）或酸处理（pH 1.0持续30分钟）破坏。盲肠静脉中几乎没有促分泌素活性。该结果支持以下假设：盲肠结扎诱导的粘液样肠病的家兔的盲肠内容物刺激了健康兔结肠外植体的粘液分泌。

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