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首页> 外文期刊>Laboratory Animal Science >OXYGENATION ACTIVITIES OF CHICKEN POLYMORPHONUCLEAR LEUKOCYTES INVESTIGATED BY SELECTIVE CHEMILUMINIGENIC PROBES
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OXYGENATION ACTIVITIES OF CHICKEN POLYMORPHONUCLEAR LEUKOCYTES INVESTIGATED BY SELECTIVE CHEMILUMINIGENIC PROBES

机译：选择性化学发光探针研究的鸡多聚核白细胞的氧合活性

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摘要

The redox metabolism of myeloperoxidase-deficient rooster (chicken) polymorphonuclear leukocytes (PMNL) was analyzed by differential chemiluminigenic probes, Chicken complement-opsonified zymosan, a phagocytosable particulate stimulus, and phorbol myristate acetate, a chemical stimulus, were used to activate the PMNL respiratory burst. The two probes used were luminol (5-amino-2,3-dihydro-1,4-phthalazinedione), a general probe of oxidase-peroxidase activities, and lucigenin (dimethylbiacridinium binitrate), a selective probe of oxidase activity, Rooster PMNLs yielded dimethylbiacridinium binitrate-dependent chemiluminescence (CL) comparable to those of myeloperoxidase-containing human PMNLs after stimulation with opsonified zymosan and to a lesser extent with phorbol myristate acetate, However, the luminol-dependent CL of opsonified zymosan or phorbol myristate acetate stimulated rooster PMNLs were approximately two orders of magnitude lower than responses observed with human PMNLs, At physiologic pH, luminol is a highly sensitive, but not specific, probe of myeloperoxidase activity, Rooster erythrocytes yielded no CL with any of the probe-stimulus combinations described, Rooster PMNL viability and oxygen were required for CL, No strong correlation could be drawn between CL responses and eosinophil leukocyte concentration, The major conclusion is that rooster PMNLs, which do not have myeloperoxidase, present a significant and reproducible oxidative burst to chemical and particulate stimuli. Although lacking in peroxidase, rooster PMNLs can still present small. luminol-dependent responses.
机译：通过差异化学发光探针，鸡补体-调理酵母聚糖，可吞噬的颗粒刺激物和佛波肉豆蔻酸酯乙酸酯（一种化学刺激物）分析了髓过氧化物酶缺陷型公鸡（鸡）多形核白细胞（PMNL）的氧化还原代谢。爆裂。所使用的两种探针是鲁米诺（5-氨基-2,3-二氢-1,4-邻苯二甲二酮），一种氧化酶-过氧化物酶活性的通用探针，以及光泽精（二甲基二甲基cri啶鎓二甲酸酯），一种氧化酶活性的选择性探针，产生了公鸡PMNL在用调理的zymosan刺激后，与含髓过氧化物酶的人PMNL的化学发光（CL）相当，而用佛波肉豆蔻酸乙酸酯刺激的程度较小，但是，受光化zymosan或佛波肉豆蔻酸酯乙酸酯刺激的公鸡PMNL的鲁米诺依赖性CL的化学发光程度较高。比人PMNLs的反应低大约两个数量级。在生理pH值下，鲁米诺是髓过氧化物酶活性的高度敏感但不是特异性的探针，在描述的任何探针-刺激组合下，公鸡红细胞均未产生CL，公鸡PMNL的存活力CL需要氧气和氧气，CL反应与嗜酸性粒细胞白细胞浓度之间没有强相关性主要结论是，没有髓过氧化物酶的公鸡PMNL对化学和微粒刺激物呈现出明显且可重现的氧化爆发。尽管缺乏过氧化物酶，但公鸡的PMNL仍可能很小。鲁米诺依赖性反应。

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