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首页> 外文期刊>Laboratory Animal Science >DEVELOPMENT OF A MURINE HYPOTHERMIA MODEL FOR STUDY OF RESPIRATORY TRACT INFLUENZA VIRUS INFECTION
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DEVELOPMENT OF A MURINE HYPOTHERMIA MODEL FOR STUDY OF RESPIRATORY TRACT INFLUENZA VIRUS INFECTION

机译：呼吸道感染流感病毒感染研究的小鼠体温过低模型的建立

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摘要

A hypothermia model was developed to predict mortality and morbidity caused by respiratory influenza virus infection in mice, To increase virulence, egg-propagated influenza A/PR/8 virus was adapted for growth in mice by four blind serial passages, The mouse-adapted influenza A virus was then used to infect groups of BALB/c mice via the intranasal route, and the 50% lethal dose (LD50) was determined, Rectal temperature of the infected mice was monitored daily, and survival rate was determined at day 14 after infection, The lowest average body temperature recorded in infected mice was approximately 10 degrees C below that in noninfected mice, In mice that developed hypothermia, with body temperature of 32 degrees C or lower, morbidity and mortality inevitably occurred, In this study, the LD50 and the 50% hypothermia-inducing dose (HID50) for mouse-adapted influenza A virus were compared and calculated to be at the same dose. These results suggest that the HID50 model could be used to predict mortality and morbidity associated with influenza virus infection in mice, This model could potentially be used to substantially reduce the time and extent of suffering inflicted on experimental animals due to viral infections, and therefore may serve as a more humane alternative to LD50 determinations.
机译：建立了一个低温模型来预测小鼠呼吸道流感病毒感染引起的死亡率和发病率。为了提高毒力，通过四次盲目的连续传代使鸡蛋繁殖的A / PR / 8流感病毒适应了小鼠的生长，适应小鼠的流感然后使用病毒通过鼻内途径感染BALB / c小鼠组，并确定50％致死剂量（LD50），每天监测感染小鼠的直肠温度，并在感染后第14天确定存活率，在感染小鼠中记录的最低平均体温比未感染小鼠低约10摄氏度。在发生体温过低且体温为32摄氏度或更低的小鼠中，不可避免地会发生发病率和死亡率。在本研究中，LD50和比较小鼠适应的甲型流感病毒的50％低温诱导剂量（HID50），并计算为相同剂量。这些结果表明，HID50模型可用于预测与小鼠流感病毒感染相关的死亡率和发病率。该模型可潜在地用于大幅减少由于病毒感染而给实验动物造成痛苦的时间和程度，因此可能作为LD50测定的更人性化选择。

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