Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly.

Smith AG; Johnson CB; Vitha

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Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly.

机译：在真核宿主中表达的植物FtsZ1和FtsZ2：GTPase活性和自组装。

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Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II filaments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca(2+).

机译：植物和藻类包含FtsZ1和FtsZ2蛋白家族，它们在质体分裂中执行特定的非冗余功能。缺乏合适的表达系统阻碍了叶绿体分裂的体外研究。在这里我们报告使用真核宿主拟南芥FtsZ1-1和FtsZ2-1的表达和纯化。确定了特定的GTP酶活性，发现FtsZ1-1与FtsZ2-1不同。纯化的蛋白质易于组装成以前未报告的组装产物，称为I型和-II型细丝。与细菌FtsZ相反，拟南芥蛋白不会在Ca（2+）存在的情况下形成捆绑的薄片。

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《FEBS letters.》 |2010年第1期|共7页
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Smith AG; Johnson CB; Vitha;
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1. Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly. [J] . Smith AG, Johnson CB, Vitha FEBS letters. . 2010,第1期

机译：在真核宿主中表达的植物FtsZ1和FtsZ2：GTPase活性和自组装。
2. The cell division gene ftsZ2 of Sinorhizobium meliloti is expressed at high levels in host plant Medicago truncatula nodules in the absence of sinI [J] . Muhamad Ali K. Shakhatreh, Jayne B. Robinson African Journal of Microbiology Research . 2012,第3期

机译：在没有sinI的情况下，苜蓿中华根瘤菌的细胞分裂基因ftsZ2在寄主植物Medi藜结节中高水平表达
3. Expressing activity of promoter elements of large intergenic region from cotton leaf curl virus in host plant* [J] . XIE Yingqiu, LIU Yule, ZHU Zhen Science in China. Series C . 2001,第1期

机译：在宿主植物中表达棉叶卷曲病毒大基因间区域的启动子元件的活性*
4. Comparative Analysis of Rab GTPases and SNAREs in Eukaryotic Genomes [C] . Akiyasu C. Yoshizawa, Shuichi Kawashima, Minoru Kanehisa Workshop on Genome Informatics . 2002

机译：真核基因组中RAB GTP酶和蛛丝的比较分析
5. Biochemical analysis of the chloroplast division proteins FtsZ1 and FtsZ2 [D] . Olson, Bradley Jesse Stanford Carnahan 2008

机译：叶绿体分裂蛋白FtsZ1和FtsZ2的生化分析
6. Colocalization of Plastid Division Proteins in the Chloroplast Stromal Compartment Establishes a New Functional Relationship between FtsZ1 and FtsZ2 in Higher Plants [O] . Rosemary S. McAndrew, John E. Froehlich, Stanislav Vitha, 2001

机译：叶绿体基质隔室中质体分裂蛋白的共定位在高等植物中建立了FtsZ1和FtsZ2之间的新功能关系
7. Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly [O] . Smith Aaron G., Johnson Carol B., Vitha Stanislav, 2010

机译：在真核宿主中表达的植物FtsZ1和FtsZ2：GTPase活性和自组装

Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly.

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