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首页> 外文期刊>Cell motility and the cytoskeleton >Stimulation-induced changes in filopodial dynamics determine the action radius of growth cones in the snail Helisoma trivolvis.
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Stimulation-induced changes in filopodial dynamics determine the action radius of growth cones in the snail Helisoma trivolvis.

机译：刺激引起的丝虫动态变化决定了蜗牛Hevolvoma trivolvis生长锥的作用半径。

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摘要

Filopodia on neuronal growth cones constantly extend and retract, thereby functioning as both sensory probes and structural devices during neuronal pathfinding. To better understand filopodial dynamics and their regulation by encounters with molecules in the environment, we investigated filopodial dynamics of identified B5 neurons from the buccal ganglion of the snail Helisoma trivolvis before and after treatment with nitric oxide (NO). We have previously demonstrated that treatment with several NO-donors caused a transient, cGMP-mediated elevation in [Ca(2+)](i), which was causally related to an increase in filopodial length and a reduction in the number of filopodia on growth cones. We demonstrate here that these effects were the result of distinct changes in filopodial dynamics. The NO-donor SIN-1 induced a general increase in filopodial motility. Filopodial elongation after treatment with SIN-1 resulted from a significant increase in the rate at which filopodia extended, as well as a significant increase in the time filopodia spent elongating. The reduction in filopodial number was caused by a significant decrease in the frequency with which new filopodia were inserted into the growth cone. With the exception of the back where filopodia appeared less motile, filopodial dynamics appeared to be mostly independent of the location on the growth cone. These results suggest that NO can regulate filopodial dynamics on migrating growth cones and might function as a messenger to adjust the action radius of a growth cone during pathfinding. Copyright 1999 Wiley-Liss, Inc.
机译：神经元生长锥上的丝足不断地伸展和收缩，从而在神经元寻路过程中既作为感觉探针又作为结构装置。为了更好地了解环境中分子遇到的丝lop动力学及其调控，我们调查了一氧化氮（NO）处理前后蜗牛三旋线虫颊神经节中识别出的B5神经元的丝状动力学。我们以前已经证明，使用几种NO供体的治疗会导致cGMP介导的[Ca（2 +）]（i）短暂升高，这与丝虫的长度增加和丝虫的数量减少有因果关系生长锥。我们在这里证明这些影响是丝虫动态变化的结果。 NO供体SIN-1引起丝虫运动性的普遍增加。用SIN-1处理后的丝状体伸长是由于丝状伪足伸展的速度显着增加以及丝状伪足消长的时间明显增加所致。丝虫数量的减少是由于新丝虫足插入生长锥的频率显着降低所致。除了丝状伪足的活动力较弱的背部外，丝虫的动力学似乎主要与生长锥上的位置无关。这些结果表明，NO可以调节迁徙的生长锥上的丝虫动态，并可能在寻路过程中充当调节生长锥作用半径的信使。版权所有1999 Wiley-Liss，Inc.

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