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首页> 外文期刊>Cell motility and the cytoskeleton >Assessment of inner dynein arm structure and possible function in ciliary and flagellar axonemes.
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Assessment of inner dynein arm structure and possible function in ciliary and flagellar axonemes.

机译：评估睫状鞭毛和鞭毛轴突中的动力蛋白内部结构和可能的功能。

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摘要

The construction and assessment of a three-dimensional computer-generated model of inner dynein arms on a 96-nm repeat unit of an axonemal doublet is described. The model is based on published electron micrographs of axonemes from Tetrahymena cilia and eel sperm, which were prepared using several different techniques: negative stain, freeze etch, and thin section. The inner arm structure is represented as three inner dynein arm complexes containing four inner dynein arms (IDAs), three dyads, and one single-headed arm, each capable of bridging the interdoublet gap. The IDA structures in the model have been correlated with the domains containing dynein heavy-chain isoforms mapped by several authors using genetic analyses of Chlamydomonas mutants. The model is consistent with micrographic evidence from axonemes of cilia and flagella from other organisms that led previously to conflicting structural interpretations. In this reconciling interpretation, the different alignments of the IDAs relative to the corresponding outer dynein arms observed in micrographs of differently prepared samples, result from the IDAs being arrested at different stages of their cycles of activity in each preparation. By interpolating between these positions of arrest, cycles of activity are proposed for each of the IDAs during which the arms attach to the neighbouring doublet microtubule and drive it tipwards.
机译：描述了在轴突双峰的96 nm重复单元上建立的三维内部动力蛋白模型的三维计算机生成模型，并进行了评估。该模型基于已发表的纤毛虫和鳗精子轴蛋白的电子显微照片，这些显微照片是使用几种不同的技术制备的：负染，冷冻蚀刻和薄切片。内臂结构表示为三个内达因臂复合物，其中包含四个内达因臂（IDA），三个二元组和一个单头臂，每个都能够弥合双倍间隙。该模型中的IDA结构已与一些作者使用衣原体突变体的遗传分析绘制的含有动力蛋白重链同工型的域相关。该模型与纤毛的轴突和其他生物的鞭毛的显微证据一致，这些证据先前导致了相互矛盾的结构解释。在这种协调的解释中，在不同制备的样品的显微照片中观察到的IDA相对于相应的外部动力蛋白臂的不同排列，是由于IDA在每种制剂中处于其活动周期的不同阶段而被阻止的。通过在这些停滞位置之间进行插值，为每个IDA提出了活动周期，在此期间，臂连接到相邻的双峰微管并向前端推动。

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