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首页> 外文期刊>Cell motility and the cytoskeleton >Higher plant cortical microtubule array analyzed in vitro in the presence of the cell wall.
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Higher plant cortical microtubule array analyzed in vitro in the presence of the cell wall.

机译：在存在细胞壁的情况下，对高等植物皮质微管阵列进行了体外分析。

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Plant morphogenesis depends on an array of microtubules in the cell cortex, the cortical array. Although the cortical array is known to be essential for morphogenesis, it is not known how the array becomes organized or how it functions mechanistically. Here, we report the development of an in vitro model that provides good access to the cortical array while preserving the array's organization and, importantly, its association with the cell wall. Primary roots of maize (Zea mays) are sectioned, without fixation, in a drop of buffer and then incubated as desired before eventual fixation. Sectioning removes cytoplasm except for a residuum comprising cortical microtubules, vesicles, and fragments of plasma membrane underlying the microtubules. The majority of the cortical microtubules remain in the cut-open cells for more than 1 h, fully accessible to the incubation solution. The growth zone or more mature tissue can be sectioned, providing access to cortical arrays that are oriented either transversely orobliquely to the long axis of the root. Using this assay, we report, first, that cortical microtubule stability is regulated by protein phosphorylation; second, that cortical microtubule stability is a function of orientation, with divergent microtubules within the array depolymerizing within minutes of sectioning; and third, that the polarity of microtubules in the cortical array is not uniform. These results suggest that the organization of the cortical array involves random nucleation followed by selective stabilization of microtubules formed at the appropriate orientation, and that the signal specifying alignment must treat orientations of +/- 180 degrees as equivalent. Cell Motil. Cytoskeleton 57:26-36, 2004.
机译：植物形态发生取决于细胞皮层中的微管阵列，即皮层阵列。尽管已知皮质阵列对于形态发生是必不可少的，但是还不知道该阵列如何组织或如何机械地起作用。在这里，我们报告了一种体外模型的开发，该模型可很好地访问皮层阵列，同时保留阵列的组织，重要的是，它与细胞壁的关联。将玉米（Zea mays）的初生根未经固定地切成一滴，放在一滴缓冲液中，然后在最终固定之前根据需要进行孵育。切片除去细胞质，除了包含皮层微管，囊泡和位于微管下方的质膜碎片的残留物。大部分皮质微管在切开的细胞中保留1小时以上，孵育溶液完全可进入。可以对生长区或更成熟的组织进行切片，以提供进入相对于根的长轴横向或倾斜定向的皮质阵列的通道。使用这种测定，我们首先报道皮质微管的稳定性受蛋白质磷酸化的调节;第二，皮层微管的稳定性是取向的函数，阵列中的发散性微管在切片后几分钟内解聚。第三，皮层阵列中微管的极性不均匀。这些结果表明，皮质阵列的组织涉及随机成核，然后选择性地稳定以适当方向形成的微管，并且指定对齐的信号必须将+/- 180度的方向视为等同。细胞动力。细胞骨架57：26-36，2004。

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