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首页> 外文期刊>Cell motility and the cytoskeleton >Curcumin inhibits cell motility and alters microfilament organization and function in prostate cancer cells.
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Curcumin inhibits cell motility and alters microfilament organization and function in prostate cancer cells.

机译：姜黄素抑制前列腺癌细胞的细胞运动并改变微丝的组织和功能。

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Curcumin is a dietary phytochemical associated with anti-tumorigenic effects, but the mechanisms by which it inhibits cancer cell growth and metastasis are not completely understood. For example, little information is available regarding the effects of curcumin on cytoskeletal organization and function. In this study, time-lapse video and immunofluorescence labeling methods were used to demonstrate that curcumin significantly alters microfilament organization and cell motility in PC-3 and LNCaP human prostate cancer cells in vitro. Curcumin rapidly arrests cell movements and subsequently alters cell shape in the highly motile PC-3 cell line, but has a less noticeable effect on the relatively immobile LNCaP cell line. Stress fibers are augmented, and the overall quantity of f-actin appears to increase in both types of cells following curcumin treatment. Cytochalasin B (CB) disrupts microfilament organization in both cell lines, and causes vigorous membrane blebbing in PC-3 cells, but not LNCaP cells. Pre-treatment of cells with curcumin suppresses changes in microfilament organization caused by CB, and blocks PC-3 membrane blebbing. At least some of the effects of curcumin appear to be mediated by protein kinase C (PKC), as treatment with the PKC inhibitor bisindolylmaleimide inhibits the ability of curcumin to block CB-induced membrane blebbing. These findings demonstrate that curcumin exerts significant effects on the actin cytoskeleton in prostate cancer cells, including altering microfilament organization and function. This is a novel observation that may represent an important mechanism by which curcumin functions as a chemopreventative agent, and as an inhibitor of angiogenesis and metastasis. Cell Motil. Cytoskeleton 58:253-268, 2004.
机译：姜黄素是一种与抗肿瘤发生作用有关的饮食植物化学物质，但其抑制癌细胞生长和转移的机制尚不完全清楚。例如，关于姜黄素对细胞骨架组织和功能的影响的信息很少。在这项研究中，延时视频和免疫荧光标记方法用于证明姜黄素在体外可显着改变PC-3和LNCaP人前列腺癌细胞中的微丝组织和细胞运动。姜黄素可迅速阻止细胞运动并随后改变高度运动的PC-3细胞系中的细胞形状，但对相对固定的LNCaP细胞系的影响较小。姜黄素处理后，两种类型的细胞中的应力纤维都增加了，肌动蛋白的总量似乎增加了。细胞松弛素B（CB）破坏两种细胞系中的微丝组织，并引起PC-3细胞（而非LNCaP细胞）剧烈的膜起泡。用姜黄素预处理细胞可抑制由CB引起的微丝组织变化，并阻止PC-3膜起泡。姜黄素的至少某些作用似乎是由蛋白激酶C（PKC）介导的，因为用PKC抑制剂比辛多尔基马来酰亚胺治疗可抑制姜黄素阻止CB诱导的膜起泡的能力。这些发现表明姜黄素对前列腺癌细胞中的肌动蛋白细胞骨架具有重要作用，包括改变微丝的组织和功能。这是一个新颖的发现，可能代表姜黄素起化学预防剂以及血管生成和转移抑制剂的作用的重要机制。细胞动力。细胞骨架58：253-268，2004。

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